Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/9253
Title: In vivo biodistribution of a humanized anti-Lewis Y monoclonal antibody (hu3S193) in MCF-7 xenografted BALB/c nude mice.
Authors: Clarke, K;Lee, Fook-Thean;Brechbiel, Martin W;Smyth, Fiona E;Old, Lloyd J;Scott, Andrew M
Affiliation: Tumour Targeting Program, Ludwig Institute for Cancer Research, Austin, Australia.
Issue Date: 1-Sep-2000
Citation: Cancer Research; 60(17): 4804-11
Abstract: The biodistribution characteristics of a humanized anti-Lewis(y) antibody (hu3S193) radiolabeled to three radioisotopes, 125I, 111In, and 90Y, were examined in a BALB/c nude mouse xenograft model of breast cancer. The immunoreactivity of both 125I- and 111In-bound hu3S193 exceeded 50% and was 20% for 90Y. In vivo, labeled antibody was shown by gamma camera imaging and immunohistochemical and autoradiographic techniques to localize to Lewis(y)-expressing breast xenografts with minimal normal tissue uptake. Maximal radioisotope uptake peaked at 48 h for all three isotopes; however, the percentage of injected dose/gram and tumor retention were greater for 111In- and 90Y-bound antibody than for 125I-bound antibody. Although immunoreactivity of 111In- and 125I-labeled hu3S193 in serum was stable over a 5-day period, the amount of unlabeled 111In in serum was lower than 125I, which together with higher tumor uptake indicates better retention of 111In-labeled hu3S193 and catabolites within the tumor cells. Superior tumor uptake and retention of 111In-labeled hu3S193 and similar blood clearance compared with 125I-labeled hu3S193, suggest that radiometals are the preferred radioisotope for this antibody-antigen system. Humanized 3S193 is a promising new construct for the targeting and potential therapy of Lewis(y)-expressing tumors.
Internal ID Number: 10987290
URI: http://ahro.austin.org.au/austinjspui/handle/1/9253
URL: http://www.ncbi.nlm.nih.gov/pubmed/10987290
Type: Journal Article
Subjects: Adenocarcinoma.immunology.metabolism.radionuclide imaging
Animals
Antibodies, Monoclonal.immunology.pharmacokinetics
Autoradiography
Breast Neoplasms.immunology.metabolism.radionuclide imaging
Drug Stability
Female
Half-Life
Humans
Immunohistochemistry
Indium Radioisotopes.diagnostic use.pharmacokinetics
Iodine Radioisotopes.diagnostic use.pharmacokinetics
Isotope Labeling
Lewis Blood-Group System.immunology
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasm Transplantation
Tissue Distribution
Transplantation, Heterologous
Tumor Cells, Cultured
Yttrium Radioisotopes.diagnostic use.pharmacokinetics
Appears in Collections:Journal articles

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