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Title: Microtitre plate and assay buffer alter detection of antiphosphatidylethanolamine antibodies in lupus anticoagulant positive plasma.
Authors: Drouvalakis, K A;Buchanan, Russell R C
Affiliation: The University of Melbourne, Department of Medicine, Austin & Repatriation Medical Centre, Heidelberg, Victoria, Australia
Issue Date: 15-May-1999
Citation: Thrombosis Research; 94(4): 205-12
Abstract: The ELISA for detection of anti-phosphatidylethanolamine antibodies in patients with lupus anticoagulant was studied. The influence of microtitre plate types, antigen concentration, antibody dilution, and buffer system was evaluated. Human albumin in the blocking buffer gave greater numbers of positive plasma samples than bovine serum. Comparison of simultaneous plasma and serum samples showed only minor variation in binding characteristics.
Internal ID Number: 10336235
Type: Journal Article
Subjects: Animals
Enzyme-Linked Immunosorbent Assay.instrumentation.standards
Lupus Coagulation Inhibitor.blood.immunology
Sensitivity and Specificity
Appears in Collections:Journal articles

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