Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/22282
Title: Vitamin C measurement in critical illness: challenges, methodologies and quality improvements.
Authors: Collie, Jake T B;Greaves, Ronda F;Jones, Oliver A H;Eastwood, Glenn M;Bellomo, Rinaldo
Affiliation: School of Medicine, University of Melbourne, Parkville, Australia
Department of Paediatrics, University of Melbourne, Parkville, Australia
Department of Intensive Care, Austin Health, Heidelberg, Australia
Australian Centre for Research on Separation Science, School of Science, RMIT University, Melbourne, Australia
School of Health and Biomedical Sciences, RMIT University, Bundoora, Australia
Dorevitch Pathology, Heidelberg, Australia
RCPAQAP - Australasian Association of Clinical Biochemists Vitamins Advisory Committee, Alexandria, Australia
Victorian Clinical Genetic Services, Murdoch Children's Research Institute, Parkville, Australia
Issue Date: 12-Dec-2019
EDate: 2019-12-12
Citation: Clinical chemistry and laboratory medicine 2019; online first: 12 December
Abstract: Background There is renewed interest in high-dose vitamin C interventions in clinical medicine due to its antioxidant properties, safe use and cost-effectiveness. Yet, randomised control trials (RCTs) employing these interventions are failing to include robust analytical methodology and proper sample handling and processing techniques. Consequently, comparisons between studies becomes impossible as there is no metrological traceability and results may be prone to pre-analytical errors. Content Through published vitamin C stability studies, method comparison papers and data from vitamin C external quality assurance programs, an assessment was made on the functionality of current methods for critically ill patient samples. Summary Data was obtained from two external quality assurance programs, two papers assessing sample stability and interlaboratory agreement and a publication on vitamin C method comparisons. A shift from spectrophotometric and enzymatic methodologies to high performance liquid chromatography (HPLC) greatly improved the variability and interlaboratory agreement. Therefore, the current analytical performance of vitamin C HPLC methodologies are acceptable for the requirements of a high-dose vitamin C RCTs. Outlook Recommendations across the total testing process of vitamin C have been provided to improve the quality of the results. The harmonisation of sample handling and processing procedures will further improve the reliability of current analytical methodologies.
URI: http://ahro.austin.org.au/austinjspui/handle/1/22282
DOI: 10.1515/cclm-2019-0912
ORCID: 0000-0001-7823-8797
0000-0002-1650-8939
PubMed URL: 31829967
Type: Journal Article
Review
Subjects: ascorbic acid
critically ill
intensive care
measurement
vitamin C
Appears in Collections:Journal articles

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