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|Title:||A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma.|
|Authors:||Stringer, Brett W;Day, Bryan W;D'Souza, Rochelle C J;Jamieson, Paul R;Ensbey, Kathleen S;Bruce, Zara C;Lim, Yi Chieh;Goasdoué, Kate;Offenhäuser, Carolin;Akgül, Seçkin;Allan, Suzanne;Robertson, Thomas;Lucas, Peter;Tollesson, Gert;Campbell, Scott;Winter, Craig;Do, Hongdo;Dobrovic, Alexander;Inglis, Po-Ling;Jeffree, Rosalind L;Johns, Terrance G;Boyd, Andrew W|
|Affiliation:||QIMR Berghofer Medical Research Institute, Brisbane, Australia|
Olivia Newton John Cancer Wellness and Research Centre, Austin Health, Heidelberg, Victoria, Australia
The University of Queensland, Brisbane, Australia
Hudson Institute of Medical Research, Clayton, Victoria, Australia
Royal Brisbane and Women's Hospital, Brisbane, Australia
|Citation:||Scientific Reports 2019; 9(1): 4902|
|Abstract:||Low-passage, serum-free cell lines cultured from patient tumour tissue are the gold-standard for preclinical studies and cellular investigations of glioblastoma (GBM) biology, yet entrenched, poorly-representative cell line models are still widely used, compromising the significance of much GBM research. We submit that greater adoption of these critical resources will be promoted by the provision of a suitably-sized, meaningfully-described reference collection along with appropriate tools for working with them. Consequently, we present a curated panel of 12 readily-usable, genetically-diverse, tumourigenic, patient-derived, low-passage, serum-free cell lines representing the spectrum of molecular subtypes of IDH-wildtype GBM along with their detailed phenotypic characterisation plus a bespoke set of lentiviral plasmids for bioluminescent/fluorescent labelling, gene expression and CRISPR/Cas9-mediated gene inactivation. The cell lines and all accompanying data are readily-accessible via a single website, Q-Cell (qimrberghofer.edu.au/q-cell/) and all plasmids are available from Addgene. These resources should prove valuable to investigators seeking readily-usable, well-characterised, clinically-relevant, gold-standard models of GBM.|
|Appears in Collections:||Journal articles|
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