Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/19454
Full metadata record
DC FieldValueLanguage
dc.contributor.authorKwong, Jason C-
dc.contributor.authorChow, Eric P F-
dc.contributor.authorStevens, Kerrie-
dc.contributor.authorStinear, Timothy P-
dc.contributor.authorSeemann, Torsten-
dc.contributor.authorFairley, Christopher K-
dc.contributor.authorChen, Marcus Y-
dc.contributor.authorHowden, Benjamin P-
dc.date2017-12-15-
dc.date.accessioned2018-09-17T01:47:13Z-
dc.date.available2018-09-17T01:47:13Z-
dc.date.issued2018-03-
dc.identifier.citationSexually transmitted infections 2018; 94(2): 151-157-
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/19454-
dc.description.abstractDrug-resistant Neisseria gonorrhoeae are now a global public health threat. Direct transmission of antibiotic-resistant gonococci between individuals has been proposed as a driver for the increased transmission of resistance, but direct evidence of such transmission is limited. Whole-genome sequencing (WGS) has superior resolution to investigate outbreaks and disease transmission compared with traditional molecular typing methods such as multilocus sequence typing (MLST) and N. gonorrhoeae multiantigen sequence (NG-MAST). We therefore aimed to systematically investigate the transmission of N. gonorrhoeae between men in sexual partnerships using WGS to compare isolates and their resistance to antibiotics at a genome level. 458 couples from a large prospective cohort of men who have sex with men (MSM) tested for gonorrhoea together between 2005 and 2014 were included, and WGS was conducted on all isolates from couples where both men were culture-positive for N. gonorrhoeae. Resistance-determining sequences were identified from genome assemblies, and comparison of isolates between and within individuals was performed by pairwise single nucleotide polymorphism and pangenome comparisons, and in silico predictions of NG-MAST and MLST. For 33 of 34 (97%; 95% CI 85% to 100%) couples where both partners were positive for gonorrhoea, the resistance-determining genes and mutations were identical in isolates from each partner (94 isolates in total). Resistance determinants in isolates from 23 of 23 (100%; 95% CI 86% to 100%) men with multisite infections were also identical within an individual. These partner and within-host isolates were indistinguishable by NG-MAST, MLST and whole genomic comparisons. These data support the transmission of antibiotic-resistant strains between sexual partners as a key driver of resistance rates in gonorrhoea among MSM. This improved understanding of the transmission dynamics of N. gonorrhoeae between sexual partners will inform treatment and prevention guidelines.-
dc.language.isoeng-
dc.subjectantibiotic resistance-
dc.subjectgonorrhoea-
dc.subjectmodes of transmission-
dc.titleWhole-genome sequencing reveals transmission of gonococcal antibiotic resistance among men who have sex with men: an observational study.-
dc.typeJournal Article-
dc.identifier.journaltitleSexually transmitted infections-
dc.identifier.affiliationVictorian Life Sciences Computation Initiative, University of Melbourne, Melbourne, Victoria, Australiaen
dc.identifier.affiliationMelbourne Sexual Health Centre, Alfred Health, Carlton, Victoria, Australiaen
dc.identifier.affiliationCentral Clinical School, Faculty of Medicine, Nursing and Health Sciences, Monash University, Melbourne, Victoria, Australiaen
dc.identifier.affiliationDepartment of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Victoria, Australiaen
dc.identifier.affiliationDoherty Applied Microbial Genomics, Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Victoria, Australiaen
dc.identifier.affiliationMicrobiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Victoria, Australiaen
dc.identifier.affiliationDepartment of Infectious Diseases, Austin Health, Heidelberg, Victoria, Australiaen
dc.identifier.doi10.1136/sextrans-2017-053287-
dc.identifier.orcid0000-0003-1766-0657en
dc.identifier.orcid0000-0003-0237-1473en
dc.identifier.pubmedid29247013-
dc.type.austinJournal Article-
dc.type.austinObservational Study-
dc.type.austinResearch Support, Non-U.S. Gov't-
local.name.researcherHowden, Benjamin P
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
crisitem.author.deptInfectious Diseases-
crisitem.author.deptInfectious Diseases-
crisitem.author.deptMicrobiology-
Appears in Collections:Journal articles
Show simple item record

Page view(s)

22
checked on Mar 28, 2024

Google ScholarTM

Check


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.