Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/19317
Title: Evolutionary origins of the emergent ST796 clone of vancomycin resistant Enterococcus faecium.
Authors: Buultjens, Andrew H;Lam, Margaret M C;Ballard, Susan;Monk, Ian R;Mahony, Andrew A;Grabsch, Elizabeth A;Grayson, M Lindsay;Pang, Stanley;Coombs, Geoffrey W;Robinson, J Owen;Seemann, Torsten;Johnson, Paul D R;Howden, Benjamin P;Stinear, Timothy P
Affiliation: Victorian Life Sciences Computation Initiative, University of Melbourne , Carlton , Victoria , Australia
Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne , Melbourne , Victoria , Australia
Microbiology Diagnostic Unit, Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne , Melbourne , Victoria , Australia
School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, Australia
Department of Microbiology, Pathwest Laboratory Medicine-WA, Fiona Stanley Hospital, Murdoch, Western Australia, Australia
Department of Infectious Diseases, Fiona Stanley Hospital, Murdoch, Western Australia, Australia
Department of Infectious Diseases, Austin Health, Heidelberg, Victoria, Australia
Department of Medicine, Austin Health, The University of Melbourne, Heidelberg, Victoria, Australia
Issue Date: 24-Jan-2017
EDate: 2017
Citation: PeerJ 2017; 5: e2916
Abstract: From early 2012, a novel clone of vancomycin resistant Enterococcus faecium (assigned the multi locus sequence type ST796) was simultaneously isolated from geographically separate hospitals in south eastern Australia and New Zealand. Here we describe the complete genome sequence of Ef_aus0233, a representative ST796 E. faecium isolate. We used PacBio single molecule real-time sequencing to establish a high quality, fully assembled genome comprising a circular chromosome of 2,888,087 bp and five plasmids. Comparison of Ef_aus0233 to other E. faecium genomes shows Ef_aus0233 is a member of the epidemic hospital-adapted lineage and has evolved from an ST555-like ancestral progenitor by the accumulation or modification of five mosaic plasmids and five putative prophage, acquisition of two cryptic genomic islands, accrued chromosomal single nucleotide polymorphisms and a 80 kb region of recombination, also gaining Tn1549 and Tn916, transposons conferring resistance to vancomycin and tetracycline respectively. The genomic dissection of this new clone presented here underscores the propensity of the hospital E. faecium lineage to change, presumably in response to the specific conditions of hospital and healthcare environments.
URI: http://ahro.austin.org.au/austinjspui/handle/1/19317
DOI: 10.7717/peerj.2916
ORCID: 0000-0001-9873-7163
PubMed URL: 28149688
ISSN: 2167-8359
Type: Journal Article
Subjects: Accessory genome
Antibiotic resistance
Comparative genomics
Enterococcus faecium
Evolution
Genome sequence
Hospital adapted
PacBio
Recombination
Vancomycin resistant
Appears in Collections:Journal articles

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