Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/18994
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dc.contributor.authorWallace, John R-
dc.contributor.authorMangas, Kirstie M-
dc.contributor.authorPorter, Jessica L-
dc.contributor.authorMarcsisin, Renee-
dc.contributor.authorPidot, Sacha J-
dc.contributor.authorHowden, Brian-
dc.contributor.authorOmansen, Till F-
dc.contributor.authorZeng, Weiguang-
dc.contributor.authorAxford, Jason K-
dc.contributor.authorJohnson, Paul D R-
dc.contributor.authorStinear, Timothy P-
dc.date2017-04-
dc.date.accessioned2018-09-12T23:57:44Z-
dc.date.available2018-09-12T23:57:44Z-
dc.date.issued2017-04-14-
dc.identifier.citationPLoS Neglected Tropical Diseases 2017; 11(4): e0005553-
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/18994-
dc.description.abstractAddressing the transmission enigma of the neglected disease Buruli ulcer (BU) is a World Health Organization priority. In Australia, we have observed an association between mosquitoes harboring the causative agent, Mycobacterium ulcerans, and BU. Here we tested a contaminated skin model of BU transmission by dipping the tails from healthy mice in cultures of the causative agent, Mycobacterium ulcerans. Tails were exposed to mosquito (Aedes notoscriptus and Aedes aegypti) blood feeding or punctured with sterile needles. Two of 12 of mice with M. ulcerans contaminated tails exposed to feeding A. notoscriptus mosquitoes developed BU. There were no mice exposed to A. aegypti that developed BU. Eighty-eight percent of mice (21/24) subjected to contaminated tail needle puncture developed BU. Mouse tails coated only in bacteria did not develop disease. A median incubation time of 12 weeks, consistent with data from human infections, was noted. We then specifically tested the M. ulcerans infectious dose-50 (ID50) in this contaminated skin surface infection model with needle puncture and observed an ID50 of 2.6 colony-forming units. We have uncovered a biologically plausible mechanical transmission mode of BU via natural or anthropogenic skin punctures.-
dc.language.isoeng-
dc.titleMycobacterium ulcerans low infectious dose and mechanical transmission support insect bites and puncturing injuries in the spread of Buruli ulcer.-
dc.typeJournal Article-
dc.identifier.journaltitlePLoS Neglected Tropical Diseases-
dc.identifier.affiliationDepartment of Internal Medicine, University of Groningen, Groningen, RB, The Netherlandsen
dc.identifier.affiliationDepartment of Infectious Diseases, Austin Health, Heidelberg, Victoria, Australiaen
dc.identifier.affiliationPest and Environmental Adaptation Research Group, Bio21 Institute and School of BioSciences, University of Melbourne, Parkville, Victoria, Australiaen
dc.identifier.affiliationDepartment of Biology, Millersville University, Millersville, PA, United States of Americaen
dc.identifier.affiliationDepartment of Microbiology and Immunology, at the Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Australiaen
dc.identifier.doi10.1371/journal.pntd.0005553-
dc.identifier.orcid0000-0001-9873-7163en
dc.identifier.orcid0000-0003-0150-123Xen
dc.identifier.pubmedid28410412-
dc.type.austinJournal Article-
local.name.researcherJohnson, Paul D R
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
crisitem.author.deptInfectious Diseases-
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