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|Title:||Development of monoclonal anti-PDGF-CC antibodies as tools for investigating human tissue expression and for blocking PDGF-CC induced PDGFRα signalling in vivo.|
|Authors:||Li, Hong;Zeitelhofer, Manuel;Nilsson, Ingrid;Liu, Xicong;Allan, Laura C;Gloria, Benjamin;Perani, Angelo;Murone, Carmel;Catimel, Bruno;Neville, A Munro;Scott, Fiona E;Scott, Andrew M;Eriksson, Ulf|
|Affiliation:||Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden|
Ludwig Institute for Cancer Research, Austin Health, Heidelberg, Victoria, Australia
Ludwig Institute for Cancer Research, New York, New York, United States of America
Olivia Newton-John Cancer Research Institute, Heidelberg, Victoria, Australia
School of Cancer Medicine, La Trobe University, Melbourne, Australia
|Citation:||PloS one 2018; 13(7): e0201089|
|Abstract:||PDGF-CC is a member of the platelet-derived growth factor (PDGF) family that stimulates PDGFRα phosphorylation and thereby activates intracellular signalling events essential for development but also in cancer, fibrosis and neuropathologies involving blood-brain barrier (BBB) disruption. In order to elucidate the biological and pathological role(s) of PDGF-CC signalling, we have generated high affinity neutralizing monoclonal antibodies (mAbs) recognizing human PDGF-CC. We determined the complementarity determining regions (CDRs) of the selected clones, and mapped the binding epitope for clone 6B3. Using the monoclonal 6B3, we determined the expression pattern for PDGF-CC in different human primary tumours and control tissues, and explored its ability to neutralize PDGF-CC-induced phosphorylation of PDGFRα. In addition, we showed that PDGF-CC induced disruption of the blood-retinal barrier (BRB) was significantly reduced upon intraperitoneal administration of a chimeric anti-PDGF-CC antibody. In summary, we report on high affinity monoclonal antibodies against PDGF-CC that have therapeutic efficacy in vivo.|
|Appears in Collections:||Journal articles|
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