Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/18743
Title: Mycoplasma Infection Alters Cancer Stem Cell Properties in Vitro.
Authors: Gedye, Craig;Cardwell, Tracy;Dimopoulos, Nektaria;Tan, Bee Shin;Jackson, Heather M;Svobodov√°, Suzanne;Anaka, Matthew;Behren, Andreas;Maher, Christopher;Hofmann, Oliver;Hide, Winston;Caballero, Otavia;Davis, Ian D;Cebon, Jonathan S
Affiliation: Department of Biostatistics, Harvard School of Public Health, 655 Huntington Avenue, SPH2, 4th Floor, Boston, MA, 02115, USA
South African National Bioinformatics Institute, University of the Western Cape, Private Bag X17, Bellville, 7535, South Africa
University of Newcastle, School of Biomedical Sciences and Pharmacy, Hunter Medical Research Institute, New Lambton Heights, NSW, 2305, Australia
Washington University Genome Institute, St. Louis, MO, 63110, USA
Ludwig Institute for Cancer Research, Austin Health, Heidelberg, Victoria, Australia
Olivia Newton-John Cancer Research Institute, Heidelberg, Victoria, Australia
Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY, 10021, USA
Eastern Health Clinical School, Faculty of Medicine, Nursing and Health Sciences, Monash University, and Eastern Health, Box Hill Hospital, 5 Arnold St, Box Hill, VIC, 3128, Australia
School of Cancer Medicine, LaTrobe University, Heidelberg, VIC, Australia
Issue Date: Feb-2016
Citation: Stem cell reviews 2016-02; 12(1): 156-61
Abstract: Cancer cell lines can be useful to model cancer stem cells. Infection with Mycoplasma species is an insidious problem in mammalian cell culture. While investigating stem-like properties in early passage melanoma cell lines, we noted poorly reproducible results from an aliquot of a cell line that was later found to be infected with Mycoplasma hyorhinis. Deliberate infection of other early passage melanoma cell lines aliquots induced variable and unpredictable effects on expression of putative cancer stem cell markers, clonogenicity, proliferation and global gene expression. Cell lines established in stem cell media (SCM) were equally susceptible. Mycoplasma status is rarely reported in publications using cultured cells to study the cancer stem cell hypothesis. Our work highlights the importance of surveillance for Mycoplasma infection while using any cultured cells to interrogate tumor heterogeneity.
URI: http://ahro.austin.org.au/austinjspui/handle/1/18743
DOI: 10.1007/s12015-015-9630-8
ORCID: 0000-0002-1629-4352
0000-0001-5329-280X
0000-0002-9066-8244
PubMed URL: 26514153
Type: Journal Article
Research Support, Non-U.S. Gov't
Subjects: Cancer cell lines, CD133
Cancer stem cells
Mycoplasma
Appears in Collections:Journal articles

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