Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/18469
Title: Functional analysis of the first complete genome sequence of a multidrug resistant sequence type 2 Staphylococcus epidermidis.
Authors: Lee, Jean Y H;Monk, Ian R;Pidot, Sacha J;Singh, Siddarth;Chua, Kyra Y L;Seemann, Torsten;Stinear, Timothy P;Howden, Benjamin P
Affiliation: Doherty Applied Microbial Genomics, Department of Microbiology & Immunology at The Doherty Institute for Infection & Immunity, University of Melbourne, Melbourne, Australia
Pacific Biosciences, Menlo Park, California, USA
Department of Infectious Diseases, Austin Health, Heidelberg, Victoria, Australia
Victorian Life Sciences Computation Inititative, University of Melbourne, Melbourne, Victoria, Australia
Department of Microbiology & Immunology at The Doherty Institute for Infection & Immunity, University of Melbourne, Melbourne, Australia
Department of Microbiology, Austin Health, Heidelberg, Victoria, Australia
Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology & Immunology at The Doherty Institute for Infection & Immunity, University of Melbourne, Melbourne, Australia
Issue Date: 20-Sep-2016
EDate: 2016-09-20
Citation: Microbial genomics 2016; 2(9): e000077
Abstract: Staphylococcus epidermidis is a significant opportunistic pathogen of humans. The ST2 lineage is frequently multidrug-resistant and accounts for most of the clinical disease worldwide. However, there are no publically available, closed ST2 genomes and pathogenesis studies have not focused on these strains. We report the complete genome and methylome of BPH0662, a multidrug-resistant, hospital-adapted, ST2 S. epidermidis, and describe the correlation between resistome and phenotype, as well as demonstrate its relationship to publically available, international ST2 isolates. Furthermore, we delineate the methylome determined by the two type I restriction modification systems present in BPH0662 through heterologous expression in Escherichia coli, allowing the assignment of each system to its corresponding target recognition motif. As the first, to our knowledge, complete ST2 S. epidermidis genome, BPH0662 provides a valuable reference for future genomic studies of this clinically relevant lineage. Defining the methylome and the construction of these E. coli hosts provides the foundation for the development of molecular tools to bypass restriction modification systems in this lineage that has hitherto proven intractable.
URI: http://ahro.austin.org.au/austinjspui/handle/1/18469
DOI: 10.1099/mgen.0.000077
ORCID: 0000-0003-0237-1473
PubMed URL: 28785416
Type: Journal Article
Research Support, Non-U.S. Gov't
Subjects: Staphylococcus epidermidis
antibiotic resistance
comparative genomics
methylome
Appears in Collections:Journal articles

Files in This Item:
There are no files associated with this item.


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.