Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/17722
Title: Target-Specific Assay for Rapid and Quantitative Detection of Mycobacterium chimaera DNA.
Authors: Zozaya-Valdés, Enrique;Porter, Jessica L;Coventry, John;Fyfe, Janet A M;Carter, Glen P;Gonçalves da Silva, Anders;Schultz, Mark B;Seemann, Torsten;Johnson, Paul D R;Stewardson, Andrew J;Bastian, Ivan;Roberts, Sally A;Howden, Benjamin P;Williamson, Deborah A;Stinear, Timothy P
Affiliation: Department of Microbiology and Immunology, The Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Victoria, Australia
Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, The Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Victoria, Australia
Victorian Infectious Diseases Laboratory, The Doherty Institute for Infection and Immunity, Victoria, Australia
Victorian Life Sciences Computational Initiative, University of Melbourne, Melbourne, Victoria, Australia
Austin Health, Heidelberg, Victoria, Australia
SA Pathology, Adelaide, South Australia, Australia
Mycobacterial Laboratory, LabPlus, Auckland, New Zealand
Doherty Applied Microbial Genomics, The Doherty Institute for Infection and Immunity, University of Melbourne, Victoria, Australia
Issue Date: Jun-2017
EDate: 2017-04-05
Citation: Journal of clinical microbiology 2017; 55(6): 1847-1856
Abstract: Mycobacterium chimaera is an opportunistic environmental mycobacterium belonging to the Mycobacterium avium-M. intracellulare complex. Although most commonly associated with pulmonary disease, there has been growing awareness of invasive M. chimaera infections following cardiac surgery. Investigations suggest worldwide spread of a specific M. chimaera clone, associated with contaminated hospital heater-cooler units used during the surgery. Given the global dissemination of this clone, its potential to cause invasive disease, and the laboriousness of current culture-based diagnostic methods, there is a pressing need to develop rapid and accurate diagnostic assays specific for M. chimaera Here, we assessed 354 mycobacterial genome sequences and confirmed that M. chimaera is a phylogenetically coherent group. In silico comparisons indicated six DNA regions present only in M. chimaera We targeted one of these regions and developed a TaqMan quantitative PCR (qPCR) assay for M. chimaera with a detection limit of 100 CFU/ml in whole blood spiked with bacteria. In vitro screening against DNA extracted from 40 other mycobacterial species and 22 bacterial species from 21 diverse genera confirmed the in silico-predicted specificity for M. chimaera Screening 33 water samples from heater-cooler units with this assay highlighted the increased sensitivity of PCR compared to culture, with 15 of 23 culture-negative samples positive by M. chimaera qPCR. We have thus developed a robust molecular assay that can be readily and rapidly deployed to screen clinical and environmental specimens for M. chimaera.
URI: http://ahro.austin.org.au/austinjspui/handle/1/17722
DOI: 10.1128/JCM.00197-17
PubMed URL: 28381604
Type: Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't
Subjects: diagnostics
genomics
infectious disease
mycobacterium
Appears in Collections:Journal articles

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