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Title: Mammographically dense human breast tissue stimulates progression to invasive lesions and metastasis
Authors: Huo, Cecilia W;Waltham, Mark;Khoo, Christine;Fox, Stephen B;Hill, Prue;Chen, Shou;Chew, Grace L;Price, John T;Nguyen, Chau H;Williams, Elizabeth D;Henderson, Michael;Thompson, Erik W;Britt, Kara L
Issue Date: 25-Oct-2016
Citation: Breast Cancer Research 2016; 18(1): 106
Abstract: BACKGROUND: High mammographic density (HMD) not only confers a significantly increased risk of breast cancer (BC) but also is associated with BCs of more advanced stages. However, it is unclear whether BC progression and metastasis are stimulated by HMD. We investigated whether patient-derived HMD breast tissue could stimulate the progression of cells compared with patient-matched low mammographic density (LMD) tissue. METHODS: Sterile breast specimens were obtained immediately after prophylactic mastectomy from high-risk women (nā€‰=ā€‰10). HMD and LMD regions of each specimen were resected under radiological guidance. Human cells, a model of ductal carcinoma in situ (DCIS), were implanted into silicone biochambers in the groins of severe combined immunodeficiency mice, either alone or with matched LMD or HMD tissue (1:1), and maintained for 6 weeks. We assessed biochamber weight as a measure of primary tumour growth, histological grade of the biochamber material, circulating tumour cells and metastatic burden by luciferase and histology. All statistical tests were two-sided. RESULTS: HMD breast tissue led to increased primary tumour take, increased biochamber weight and increased proportions of high-grade DCIS and grade 3 invasive BCs compared with LMD. This correlated with an increased metastatic burden in the mice co-implanted with HMD tissue. CONCLUSIONS: Our study is the first to explore the direct effect of HMD and LMD human breast tissue on the progression and dissemination of BC cells in vivo. The results suggest that HMD status should be a consideration in decision-making for management of patients with DCIS lesions.
DOI: 10.1186/s13058-016-0767-4
PubMed URL:
Type: Journal Article
Subjects: Breast cancer
Mammographic density
Murine biochamber
Appears in Collections:Journal articles

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