Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/13634
Title: Angiotensin converting enzyme inhibition reduces the expression of transforming growth factor-beta1 and type IV collagen in diabetic vasculopathy.
Authors: Rumble, J R;Gilbert, Richard E;Cox, Allison J;Wu, L;Cooper, Mark E
Affiliation: Department of Medicine, University of Melbourne, Austin & Repatriation Medical Centre, Heidelberg, Australia.
Issue Date: 1-Nov-1998
Citation: Journal of Hypertension; 16(11): 1603-9
Abstract: The purpose of this study was to assess the role of transforming growth factor (TGF)-beta1 in the development of diabetes-associated mesenteric vascular hypertrophy and in the antitrophic effect of angiotensin converting enzyme inhibitors.Streptozotocin-induced diabetic and control Sprague-Dawley rats were randomly allocated to treatment with the angiotensin converting enzyme inhibitor ramipril or to no treatment and were killed 1 or 3 weeks after the streptozotocin injection. Blood was collected and mesenteric vessels removed. Mesenteric vascular weight was measured and TGF-beta1 and alpha1 (type IV) collagen messenger (m)RNA levels were analysed by Northern analysis. Immunohistochemical analyses for TGF-beta1 and type IV collagen were also performed.The diabetic rats had increased mesenteric vessel weight at 3 weeks but not at 1 week and a concomitant rise in mesenteric TGF-beta1 and in alpha1 (type IV) collagen mRNA levels. Ramipril treatment attenuated mesenteric vessel hypertrophy and prevented the increase in TGF-beta1 and alpha1 (type IV) collagen mRNA levels after 3 weeks of diabetes. The immunohistochemical analysis revealed that diabetes was associated with increased TGF-beta1 and type IV collagen protein and extracellular matrix accumulation in mesenteric vessels, and this increase was reduced by ramipril treatment.These results support the concept that TGF-beta is involved in the changes associated with diabetic vascular disease, and suggest a mechanism by which angiotensin converting enzyme inhibitors exert their antitrophic effects.
Internal ID Number: 9856360
URI: http://ahro.austin.org.au/austinjspui/handle/1/13634
URL: http://www.ncbi.nlm.nih.gov/pubmed/9856360
Type: Journal Article
Subjects: Angiotensin-Converting Enzyme Inhibitors.pharmacology
Animals
Blood Glucose.drug effects
Blood Pressure.drug effects
Blotting, Northern
Body Weight.drug effects
Collagen.genetics
Diabetes Mellitus, Experimental.complications
Diabetic Angiopathies.complications.genetics.metabolism
Gene Expression.drug effects
Immunohistochemistry
Male
RNA, Messenger.analysis.genetics
Ramipril.pharmacology
Rats
Rats, Sprague-Dawley
Transforming Growth Factor beta.genetics
Vascular Diseases.complications.genetics.metabolism
Appears in Collections:Journal articles

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