Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/13608
Title: In vitro characterization of a recombinant 32P-phosphorylated anti-(carcinoembryonic antigen) single-chain antibody.
Authors: Patrick, M R;Chester, K A;Pietersz, Geoffrey A
Affiliation: Austin Research Institute, Austin Hospital, Heidelberg, Victoria, Australia.
Issue Date: 1-Jun-1998
Citation: Cancer Immunology, Immunotherapy : Cii; 46(4): 229-37
Abstract: The major limitations of monoclonal antibody conjugates as therapeutic agents have been their poor tumour targeting, inadequate tumour penetration and immunogenicity. More even and deeper tissue penetration has been demonstrated with smaller antibody fragments. The smaller size and absence of an Fc segment may contribute to a lowered immunogenicity with single-chain antibodies (scFv) and also permit their recombinant engineering and bacterial expression. We describe the successful engineering, expression and pre-clinical characterisation of a phosphorylatable "kemptide" (Leu-Arg-Arg-Ala-Ser-Gly) anti-carcinoembryonic antigen (anti-CEA) scFv (PKS-scFv), for use as a radioimmunotherapeutic agent. Specifically, a yield of 6 mg/l induced culture was obtained. Site-specific phosphorylation was demonstrated without loss of specificity. In vitro assays revealed a selective cytotoxicity of 32P-PKS-scFv for high-CEA-expressing LS-174T cells compared to the low-CEA-expressing HT-29 cells, with a rapid internalisation rate.
Internal ID Number: 9671146
URI: http://ahro.austin.org.au/austinjspui/handle/1/13608
URL: http://www.ncbi.nlm.nih.gov/pubmed/9671146
Type: Journal Article
Subjects: Binding Sites
Carcinoembryonic Antigen.chemistry.immunology.metabolism
Cloning, Molecular
Colonic Neoplasms.metabolism.radiotherapy
Drug Stability
Escherichia coli.genetics.metabolism
Genetic Engineering.methods
Humans
Immunoglobulin Fragments.biosynthesis.chemistry.genetics
Immunoglobulin Variable Region.biosynthesis.chemistry.genetics
Immunotoxins.chemistry
Oligopeptides.chemistry
Phosphorus Radioisotopes.chemistry
Phosphorylation
Radioimmunotherapy
Recombinant Proteins.biosynthesis.chemistry.genetics
Tumor Cells, Cultured
Appears in Collections:Journal articles

Files in This Item:
There are no files associated with this item.


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.