Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/13550
Title: Localization of bradykinin B2 binding sites in rat kidney following chronic ACE inhibitor treatment.
Authors: Dean, Rachael G;Murone, Carmel;Lew, R A;Zhuo, J;Casley, David J;Müller-Esterl, W;Alcorn, D;Mendelsohn, Frederick AO
Affiliation: University of Melbourne, Department of Medicine, Austin, Heidelberg, Australia.
Issue Date: 1-Nov-1997
Citation: Kidney International; 52(5): 1261-70
Abstract: Bradykinin exerts important influences on renal hemodynamics and tubular function by acting on renal bradykinin B2 receptors. However, the precise sites and mechanisms of its actions on the kidney are not known. To help elucidate the mechanisms of renal actions of bradykinin in vivo, we have employed high resolution electron microscopic autoradiography to localize bradykinin B2 binding sites in the rat kidney following intravenous administration of a radiolabeled ligand, 125I-HPP-Hoe140 (3-4-Hydroxyphenyl-propionyl-DArg0-[Hyp3-Thi5-D-Tic 7-Oic8]-bradykinin), a derivative of the highly selective bradykinin B2 receptor antagonist, Hoe140. In non-treated rats, bradykinin B2 binding sites were localized to the cell bodies and the luminal brush border of the proximal convoluted tubules in the cortex. In the medulla (except for the outer stripe of the outer medulla), binding occurred in the distal tubules, thin limbs of the loop of Henle, collecting ducts, peritubular capillary endothelium and renomedullary interstitial cells. To exclude the possibility that the radioligand may bind to angiotensin converting enzyme, rats were pretreated with the angiotensin converting enzyme inhibitor, perindopril. In these rats, binding to the cell bodies and the luminal brush border of the proximal convoluted tubules in the cortex was completely abolished, while binding remained unaltered in the medulla. Further studies using high performance liquid chromatography revealed that while the radioligand was degraded following systemic administration in nontreated rats, the degradation was significantly reduced in the rats pretreated chronically with perindopril. These results indicate that binding detected in the proximal tubules in the normal rats is due primarily to the tubular uptake of the degraded radioligand, and that bradykinin B2 binding sites occur predominantly in the renal tubules, vascular endothelium, and renomedullary interstitial cells of the renal medulla.
Internal ID Number: 9350649
URI: http://ahro.austin.org.au/austinjspui/handle/1/13550
URL: http://www.ncbi.nlm.nih.gov/pubmed/9350649
Type: Journal Article
Subjects: Angiotensin-Converting Enzyme Inhibitors.pharmacology
Animals
Autoradiography
Binding Sites
Bradykinin.analogs & derivatives.metabolism
Iodine Radioisotopes.diagnostic use
Kidney.chemistry
Male
Rats
Rats, Sprague-Dawley
Receptor, Bradykinin B2
Receptors, Bradykinin.analysis
Appears in Collections:Journal articles

Files in This Item:
There are no files associated with this item.


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.