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Title: Nitric oxide synthase in neurons of the human gall-bladder and its colocalization with neuropeptides.
Authors: Uemura, S;Pompolo, S;Furness, J B;Hardy, Kenneth John
Affiliation: Department of Anatomy and Cell Biology, University of Melbourne, Austin Hospital, Heidelberg, Victoria, Australia.
Issue Date: 1-Mar-1997
Citation: Journal of Gastroenterology and Hepatology; 12(3): 257-65
Abstract: The distributions of nerve cells and fibres that are immunoreactive for nitric oxide synthase (NOS) have been investigated in the human gall-bladder. In addition, the colocalization of NOS immunoreactivity (IR) with neuropeptide Y (NPY), pituitary adenylyl cyclase activating peptide (PACAP), somatostatin (SOM), substance P (SP), tyrosine hydroxylase (TH) and vasoactive intestinal peptide (VIP)-IR was determined. Nitric oxide synthase-IR nerve cell bodies comprised 13 and 30% of nerve cells in ganglia of the fibromuscular and subepithelial layers, respectively. To determine these percentages, neuron-specific enolase-IR was used as a marker for all nerve cells. Although SOM- and VIP-IR nerve cell bodies were found in both ganglia, they rarely contained NOS-IR. In the fibromuscular layer, NOS-IR nerve fibres were abundant and most PACAP-, SOM- and VIP-IR fibres and many NPY-IR fibres were also NOS positive. No colocalization was observed between NOS- and SP- or TH-IR. In the mucosal layer, moderate numbers of NOS-IR fibres were found and the degree of colocalization of NOS-IR with each of NPY-, PACAP-, SOM-, SP- and VIP-IR were as follows: PACAP and NPY > VIP > SOM and SP. Nitric oxide synthase and TH were not colocalized in mucosal fibres. These results suggest that nerve fibres in the fibromuscular layer in the human gall-bladder with the chemical coding NOS/NPY/PACAP/SOM/VIP are axons of inhibitory motor neurons. Nitric oxide synthase-IR fibres in the mucosal layer that contained NPY, PACAP, SOM, SP and VIP with various degrees of colocalization probably contribute to the control of epithelial secretion or absorption.
Internal ID Number: 9142645
Type: Journal Article
Subjects: Gallbladder.innervation
Ganglia.anatomy & histology
Nerve Fibers.ultrastructure
Nitric Oxide Synthase.metabolism
Phosphopyruvate Hydratase.metabolism
Tissue Distribution
Appears in Collections:Journal articles

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