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Title: Quantification of serum proteins using capillary electrophoresis.
Authors: Jenkins, Margaret A;Guerin, M D
Affiliation: Department of Chemical Pathology, Heidelberg Repatriation Hospital, Victoria, Australia.
Issue Date: 1-Sep-1995
Citation: Annals of Clinical Biochemistry; 32 ( Pt 5)(): 493-7
Abstract: Capillary electrophoresis is a technique that can be automated for the separation of charged particles. By investigating suitable sample dilution and injection time and adhering to a strict washing procedure we have been able to quantify paraproteins in serum samples. This has enabled us to use the technique of capillary electrophoresis for the provision of serum protein electrophoresis in a routine clinical laboratory. We present our findings of 260 serum samples, which included 76 samples with paraproteins analysed by both capillary electrophoresis (EC) and high resolution agarose gel electrophoresis (HRAGE). CE was able to detect all the monoclonal bands detected by HRAGE, and in particular, better able to detect IgA monoclonal bands occurring in the beta region. The major advantages of CE over HRAGE relate to the automated nature of CE with the elimination of the need for a densitometer.
Internal ID Number: 8830625
Type: Journal Article
Subjects: Blood Protein Electrophoresis.methods
Blood Proteins.analysis
Electrophoresis, Agar Gel
Electrophoresis, Capillary.methods
Appears in Collections:Journal articles

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