Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/13389
Title: Detection of Clostridium difficile enterotoxin gene in clinical specimens by the polymerase chain reaction.
Authors: Boondeekhun, H S;Gurtler, V;Odd, M L;Wilson, V A;Mayall, Barrie C
Affiliation: Department of Microbiology, Heidelberg Repatriation Hospital, Victoria, Australia.
Issue Date: 1-May-1993
Citation: Journal of Medical Microbiology; 38(5): 384-7
Abstract: A rapid assay was developed for detection of the Clostridium difficile enterotoxin gene in stool specimens by means of the polymerase chain reaction (PCR). The PCR primers amplified a 63-bp repetitive sequence of the enterotoxin gene, thereby generating a distinctive ladder pattern of DNA bands following electrophoresis. Crude DNA extracts from stools containing C. difficile produced one (63-bp) or more bands of the characteristic ladder. Of 172 stool specimens from 58 patients, 37 gave positive results by culture (15 specimens) or cytotoxin assay (36 specimens). When 36 available "positive" specimens were tested by the PCR assay, 34 (94%) gave positive results--24 by direct testing, and 10 after extraction of DNA by the Qiagen procedure. Insufficient material of the remaining two specimens was available for DNA extraction. Of 21 stools "negative" for C. difficile by culture or cytotoxin assay, one gave a positive result by PCR and seven produced atypical bands. The rapid PCR detection technique for C. difficile was more sensitive than standard culture, and of a sensitivity similar to cytotoxin testing. The method has the potential for adoption in routine laboratory practice.
Internal ID Number: 8487296
URI: http://ahro.austin.org.au/austinjspui/handle/1/13389
URL: http://www.ncbi.nlm.nih.gov/pubmed/8487296
Type: Journal Article
Subjects: Bacterial Toxins
Clostridium difficile.genetics
Enterocolitis, Pseudomembranous.microbiology
Enterotoxins.genetics
Feces.microbiology
Genes, Bacterial
Humans
Polymerase Chain Reaction.methods
Appears in Collections:Journal articles

Files in This Item:
There are no files associated with this item.


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.