Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/13307
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dc.contributor.authorWiley, J Sen
dc.contributor.authorCebon, Jonathan Sen
dc.contributor.authorJamieson, Gary Pen
dc.contributor.authorSzer, Jen
dc.contributor.authorGibson, Jen
dc.contributor.authorWoodruff, R Ken
dc.contributor.authorMcKendrick, J Jen
dc.contributor.authorSheridan, W Pen
dc.contributor.authorBiggs, J Cen
dc.contributor.authorSnook, M Ben
dc.date.accessioned2015-05-16T03:08:05Z
dc.date.available2015-05-16T03:08:05Z
dc.date.issued1994-01-01en
dc.identifier.citationLeukemia; 8(1): 181-5en
dc.identifier.govdoc8289485en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/13307en
dc.description.abstractNucleoside transporter expression has been linked to proliferation in a variety of haemopoietic cell types. Granulocyte-macrophage colony-stimulating factor (GM-CSF) was given for 72 h before commencing chemotherapy in 15 patients with relapsed or refractory acute myeloid leukaemia (AML) and in 11 patients serial bone marrows were taken for measurement of [3H]thymidine labelling index, Ki-67 positivity and maximal binding of 5-(SAENTA-x8)-fluorescein, a flow cytometry ligand which enumerates nucleoside transporter sites. GM-CSF caused proliferation of marrow myeloblasts in eight of 11 patients, while in three patients there was no change in proliferative indices. The expression of nucleoside transporters increased up to 4-fold in the myeloblasts from the patients showing a proliferative response to GM-CSF but there was no increase in transporters on the myeloblasts from the three non-responding patients. A close correlation was found between the fold increase in nucleoside transporter expression and the fold increase in labelling index of marrow myeloblasts (r = 0.86, n = 9, p < 0.01). In one patient with acute megakaryoblastic leukemia, GM-CSF caused parallel increases in labelling index, Ki-67 positivity and numbers of nucleoside transporters on peripheral blood blast cells. Thus induction of proliferation by cytokine increases the expression of nucleoside transporters on leukaemic myeloblasts studied in serial samples from the same source (bone marrow or blood). The suitability of 5-(SAENTA-x8)-fluorescein for two colour flow cytometric analysis allows the rapid enumeration of nucleoside transporters in the myeloblast compartment of heterogeneous marrow samples.en
dc.language.isoenen
dc.subject.otherAcute Diseaseen
dc.subject.otherBlood Proteins.analysisen
dc.subject.otherBone Marrow.drug effectsen
dc.subject.otherBone Marrow Cellsen
dc.subject.otherCarrier Proteins.analysisen
dc.subject.otherCell Division.drug effectsen
dc.subject.otherFluoresceinsen
dc.subject.otherGranulocyte-Macrophage Colony-Stimulating Factor.therapeutic useen
dc.subject.otherHumansen
dc.subject.otherLeukemia, Megakaryoblastic, Acute.drug therapy.pathologyen
dc.subject.otherLeukemia, Myeloid.drug therapy.pathologyen
dc.subject.otherMembrane Proteins.analysisen
dc.subject.otherNucleoside Transport Proteinsen
dc.subject.otherPurine Nucleosidesen
dc.subject.otherThymidine.metabolismen
dc.subject.otherTritiumen
dc.titleAssessment of proliferative responses to granulocyte-macrophage colony-stimulating factor (GM-CSF) in acute myeloid leukaemia using a fluorescent ligand for the nucleoside transporter.en
dc.typeJournal Articleen
dc.identifier.journaltitleLeukemiaen
dc.identifier.affiliationDepartment of Haematology, Austin Hospital, Victoria, Australiaen
dc.description.pages181-5en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/8289485en
dc.type.austinJournal Articleen
local.name.researcherCebon, Jonathan S
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
crisitem.author.deptOlivia Newton-John Cancer Research Institute-
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