Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/13127
Title: Induction of Ca2+ signaling and possible exocytosis in endothelial cells by a stable leukocyte-derived factor.
Authors: Liu, J J;Chen, J R;Bradley, C J;Johnston, Colin I;Buxton, Brian F
Affiliation: University of Melbourne, Austin Hospital, Department of Cardiac Surgery, Heidelberg, Vic, Australia.
Issue Date: 5-Jan-1995
Citation: Biochemical and Biophysical Research Communications; 206(1): 146-52
Abstract: Previous studies have shown that polymorphonuclear leukocytes (PMNs) release a stable factor that inhibits endothelium-dependent relaxation. In the present studies, the effects of the factor on Ca2+ signaling and on ultrastructure of endothelial cells were investigated. In the cultured endothelial cells, the PMN-derived factor induced an increase in [Ca2+]i in a pattern of oscillations. The frequency of the Ca2+ oscillations was less than 3 spikes/10 minutes. Removal of extracellular Ca2+ by perfusion with Ca(2+)-free Krebs' solution abolished the spikes. The results of electron microscopy showed that this factor induced an increase in vesicle formation on the luminal surface of the rat aortic endothelium. The increased vesicle formation may represent exocytosis. The structure of the smooth muscle cells was not changed. In conclusion, the PMN-derived factor induces a Ca2+ influx and In conclusion, the PMN-derived factor induces a Ca2+ influx and possible exocytosis, suggesting that the factor may have other biological functions besides the inhibition of the vascular relaxation.
Internal ID Number: 7818514
URI: http://ahro.austin.org.au/austinjspui/handle/1/13127
DOI: 10.1006/bbrc.1995.1021
URL: http://www.ncbi.nlm.nih.gov/pubmed/7818514
Type: Journal Article
Subjects: Animals
Aorta
Biological Factors.isolation & purification.pharmacology
Calcium.metabolism.pharmacology
Cells, Cultured
Cytosol.metabolism
Endothelium, Vascular.drug effects.physiology.ultrastructure
Exocytosis.drug effects
Humans
In Vitro Techniques
Kinetics
Microscopy, Electron
Neutrophils.physiology
Norepinephrine.pharmacology
Oscillometry
Perfusion
Rats
Signal Transduction.drug effects
Time Factors
Appears in Collections:Journal articles

Files in This Item:
There are no files associated with this item.


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.