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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chen, J R | en |
dc.contributor.author | Jamieson, Gary P | en |
dc.contributor.author | Wiley, J S | en |
dc.date.accessioned | 2015-05-16T02:47:53Z | |
dc.date.available | 2015-05-16T02:47:53Z | |
dc.date.issued | 1994-08-15 | en |
dc.identifier.citation | Biochemical and Biophysical Research Communications; 202(3): 1511-6 | en |
dc.identifier.govdoc | 7520239 | en |
dc.identifier.other | PUBMED | en |
dc.identifier.uri | https://ahro.austin.org.au/austinjspui/handle/1/13024 | en |
dc.description.abstract | The permeability of lymphocytes to NH4+ was examined by measuring intracellular pH using the fluorescent pH-sensitive dye BCECF. Addition of 20 mM NH4Cl produced a rapid phase of alkalinization. This was followed by a slow return to resting pHi due to NH4+ influx. The rate of NH4+ was increased many fold by extracellular ATP and the increment showed features consistent with NH4+ being a permeant for the P2Z purinoceptor operated ion channel. Cytosolic pH measurements showed monomethylammonium+ and dimethylammonium+ were also permeants, but trimethylammonium+ (69 Dalton) was excluded by this channel. Since our previous data showed ethidium+ (314 Dalton) is a permeant it appears that molecular conformation rather than molecular weight determines entry of cationic solutes through the channel. | en |
dc.language.iso | en | en |
dc.subject.other | Adenosine Triphosphate.physiology | en |
dc.subject.other | Amiloride.analogs & derivatives.pharmacology | en |
dc.subject.other | Amines.pharmacology | en |
dc.subject.other | Biological Transport | en |
dc.subject.other | Calcium.metabolism | en |
dc.subject.other | Cell Membrane Permeability | en |
dc.subject.other | Fluoresceins | en |
dc.subject.other | Fluorescent Dyes | en |
dc.subject.other | Humans | en |
dc.subject.other | Hydrogen-Ion Concentration | en |
dc.subject.other | Ion Channels.physiology | en |
dc.subject.other | Lymphocytes.metabolism | en |
dc.subject.other | Oxidation-Reduction | en |
dc.subject.other | Quaternary Ammonium Compounds.metabolism | en |
dc.subject.other | Receptors, Purinergic.physiology | en |
dc.title | Extracellular ATP increases NH4+ permeability in human lymphocytes by opening a P2Z purinoceptor operated ion channel. | en |
dc.type | Journal Article | en |
dc.identifier.journaltitle | Biochemical and biophysical research communications | en |
dc.identifier.affiliation | Department of Haematology, Austin Hospital, Heidelberg, Victoria, Australia | en |
dc.identifier.doi | 10.1006/bbrc.1994.2102 | en |
dc.description.pages | 1511-6 | en |
dc.relation.url | https://pubmed.ncbi.nlm.nih.gov/7520239 | en |
dc.type.austin | Journal Article | en |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.fulltext | No Fulltext | - |
item.grantfulltext | none | - |
item.languageiso639-1 | en | - |
item.openairetype | Journal Article | - |
Appears in Collections: | Journal articles |
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