Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/13024
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dc.contributor.authorChen, J Ren
dc.contributor.authorJamieson, Gary Pen
dc.contributor.authorWiley, J Sen
dc.date.accessioned2015-05-16T02:47:53Z
dc.date.available2015-05-16T02:47:53Z
dc.date.issued1994-08-15en
dc.identifier.citationBiochemical and Biophysical Research Communications; 202(3): 1511-6en
dc.identifier.govdoc7520239en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/13024en
dc.description.abstractThe permeability of lymphocytes to NH4+ was examined by measuring intracellular pH using the fluorescent pH-sensitive dye BCECF. Addition of 20 mM NH4Cl produced a rapid phase of alkalinization. This was followed by a slow return to resting pHi due to NH4+ influx. The rate of NH4+ was increased many fold by extracellular ATP and the increment showed features consistent with NH4+ being a permeant for the P2Z purinoceptor operated ion channel. Cytosolic pH measurements showed monomethylammonium+ and dimethylammonium+ were also permeants, but trimethylammonium+ (69 Dalton) was excluded by this channel. Since our previous data showed ethidium+ (314 Dalton) is a permeant it appears that molecular conformation rather than molecular weight determines entry of cationic solutes through the channel.en
dc.language.isoenen
dc.subject.otherAdenosine Triphosphate.physiologyen
dc.subject.otherAmiloride.analogs & derivatives.pharmacologyen
dc.subject.otherAmines.pharmacologyen
dc.subject.otherBiological Transporten
dc.subject.otherCalcium.metabolismen
dc.subject.otherCell Membrane Permeabilityen
dc.subject.otherFluoresceinsen
dc.subject.otherFluorescent Dyesen
dc.subject.otherHumansen
dc.subject.otherHydrogen-Ion Concentrationen
dc.subject.otherIon Channels.physiologyen
dc.subject.otherLymphocytes.metabolismen
dc.subject.otherOxidation-Reductionen
dc.subject.otherQuaternary Ammonium Compounds.metabolismen
dc.subject.otherReceptors, Purinergic.physiologyen
dc.titleExtracellular ATP increases NH4+ permeability in human lymphocytes by opening a P2Z purinoceptor operated ion channel.en
dc.typeJournal Articleen
dc.identifier.journaltitleBiochemical and biophysical research communicationsen
dc.identifier.affiliationDepartment of Haematology, Austin Hospital, Heidelberg, Victoria, Australiaen
dc.identifier.doi10.1006/bbrc.1994.2102en
dc.description.pages1511-6en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/7520239en
dc.type.austinJournal Articleen
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
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