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|Title:||Extracellular ATP increases NH4+ permeability in human lymphocytes by opening a P2Z purinoceptor operated ion channel.|
|Authors:||Chen, J R;Jamieson, Gary P;Wiley, J S|
|Affiliation:||Department of Haematology, Austin Hospital, Heidelberg, Vic., Australia.|
|Citation:||Biochemical and Biophysical Research Communications; 202(3): 1511-6|
|Abstract:||The permeability of lymphocytes to NH4+ was examined by measuring intracellular pH using the fluorescent pH-sensitive dye BCECF. Addition of 20 mM NH4Cl produced a rapid phase of alkalinization. This was followed by a slow return to resting pHi due to NH4+ influx. The rate of NH4+ was increased many fold by extracellular ATP and the increment showed features consistent with NH4+ being a permeant for the P2Z purinoceptor operated ion channel. Cytosolic pH measurements showed monomethylammonium+ and dimethylammonium+ were also permeants, but trimethylammonium+ (69 Dalton) was excluded by this channel. Since our previous data showed ethidium+ (314 Dalton) is a permeant it appears that molecular conformation rather than molecular weight determines entry of cationic solutes through the channel.|
|Internal ID Number:||7520239|
Amiloride.analogs & derivatives.pharmacology
Cell Membrane Permeability
Quaternary Ammonium Compounds.metabolism
|Appears in Collections:||Journal articles|
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