Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/13024
Title: Extracellular ATP increases NH4+ permeability in human lymphocytes by opening a P2Z purinoceptor operated ion channel.
Authors: Chen, J R;Jamieson, Gary P;Wiley, J S
Affiliation: Department of Haematology, Austin Hospital, Heidelberg, Vic., Australia.
Issue Date: 15-Aug-1994
Citation: Biochemical and Biophysical Research Communications; 202(3): 1511-6
Abstract: The permeability of lymphocytes to NH4+ was examined by measuring intracellular pH using the fluorescent pH-sensitive dye BCECF. Addition of 20 mM NH4Cl produced a rapid phase of alkalinization. This was followed by a slow return to resting pHi due to NH4+ influx. The rate of NH4+ was increased many fold by extracellular ATP and the increment showed features consistent with NH4+ being a permeant for the P2Z purinoceptor operated ion channel. Cytosolic pH measurements showed monomethylammonium+ and dimethylammonium+ were also permeants, but trimethylammonium+ (69 Dalton) was excluded by this channel. Since our previous data showed ethidium+ (314 Dalton) is a permeant it appears that molecular conformation rather than molecular weight determines entry of cationic solutes through the channel.
Internal ID Number: 7520239
URI: http://ahro.austin.org.au/austinjspui/handle/1/13024
DOI: 10.1006/bbrc.1994.2102
URL: http://www.ncbi.nlm.nih.gov/pubmed/7520239
Type: Journal Article
Subjects: Adenosine Triphosphate.physiology
Amiloride.analogs & derivatives.pharmacology
Amines.pharmacology
Biological Transport
Calcium.metabolism
Cell Membrane Permeability
Fluoresceins
Fluorescent Dyes
Humans
Hydrogen-Ion Concentration
Ion Channels.physiology
Lymphocytes.metabolism
Oxidation-Reduction
Quaternary Ammonium Compounds.metabolism
Receptors, Purinergic.physiology
Appears in Collections:Journal articles

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