Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/13021
Title: Androgen manipulation and vasopressin binding in the rat brain and peripheral organs.
Authors: Gao, X;Phillips, P A;Oldfield, B;Trinder, D;Risvanis, John;Stephenson, J;Johnston, Colin I
Affiliation: Department of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Victoria, Australia.
Issue Date: 1-Mar-1994
Citation: European Journal of Endocrinology / European Federation of Endocrine Societies; 130(3): 291-6
Abstract: It is now widely recognized that there is a sexual dimorphism in the development of arginine vasopressin (AVP) immunoreactivity in certain parts of the brain, and that changes in brain AVP immunoreactivity change with manipulation of androgen status. The aim of this experiment was to determine specifically any AVP receptor changes in response to manipulation of androgen levels using a selective V1 antagonist radioligand. Following castration, plasma testosterone levels fell and AVP immunoreactivity was reduced in the lateral septum and bed nucleus of the stria terminalis. With testosterone supplementation in castrated animals, the immunoreactivity in these regions was restored to a higher degree than in sham-operated animals. Central and peripheral V1 AVP receptor binding (as determined using the selective AVP V1 antagonist radioligand [125I](d(CH2)5,sarcosine7)AVP was not changed in any of the brain regions studied or in liver or kidney membranes from the three groups. This study demonstrates that there is no change in brain AVP receptor binding despite changes in regional AVP immunoreactivity in the brain, and excludes any confounding interaction with changes in oxytocin receptors.
Internal ID Number: 7512406
URI: http://ahro.austin.org.au/austinjspui/handle/1/13021
URL: http://www.ncbi.nlm.nih.gov/pubmed/7512406
Type: Journal Article
Subjects: Androgens.analysis.blood.physiology
Animals
Arginine Vasopressin.blood.metabolism
Autoradiography
Binding Sites
Brain.metabolism.ultrastructure
Cell Membrane.chemistry.metabolism.ultrastructure
Immunohistochemistry
Iodine Radioisotopes
Kidney.chemistry.metabolism.ultrastructure
Liver.chemistry.metabolism.ultrastructure
Male
Rats
Rats, Sprague-Dawley
Receptors, Vasopressin.analysis.metabolism
Sarcosine
Testosterone.blood
Appears in Collections:Journal articles

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