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|Title:||Combined high-performance liquid chromatographic procedure for measuring 4-hydroxypropranolol and propranolol in plasma: pharmacokinetic measurements following conventional and slow-release propranolol administration.|
|Authors:||Drummer, Olaf H;McNeil, J;Pritchard, E;Louis, William J|
|Affiliation:||Clinical Pharmacology & Therapeutics Unit, Austin Hospital, University of Melbourne, Heidelberg, Victoria, Australia.|
|Citation:||Journal of Pharmaceutical Sciences; 70(9): 1030-2|
|Abstract:||An assay is described for the simultaneous determination of propranolol and its active metabolite, 4-hydroxypropranolol, in human plasma. Both compounds were separated from an ethereal extract by high-performance liquid chromatography employing a C18 bonded-phase column. Detection of the effluent was by fluorescence. Suitable fluorescent spectrometers and wavelength settings that allow optimum detection of both compounds have been described. The limit of sensitivity was 2 ng/ml for both propranolol and 4-hydroxypropranolol. Mean peak plasma levels of propranolol and 4-hydroxypropranolol in six patients receiving a single dose of a slow-release 160-mg formulation of propranolol were 28 and 6 ng/ml, respectively. These levels were about one-tenth the level obtained following a single conventionally prepared dose of propranolol (160 mg). Peak levels were delayed and plasma levels of propranolol persisted for a longer period with the slow-release formulation. Area under the curve estimates suggested that the bioavailability of the slow-release formulation following single-dose administration was about one-third that of the conventional preparation.|
|Internal ID Number:||6101148|
Chromatography, High Pressure Liquid
Propranolol.administration & dosage.analogs & derivatives.blood.pharmacokinetics
|Appears in Collections:||Journal articles|
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