Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/13000
Title: Concentrations of histamine in the hypothalamus of the rat: effect of extraction volume and interpretation of the effects of acutely-administered morphine.
Authors: Lewis, S J;Verberne, Anthony J M;Fennessy, M R;Jarrott, B
Affiliation: University of Melbourne, Department of Medicine, Austin Hospital, Heidelberg, Victoria, Australia.
Issue Date: 1-Aug-1987
Citation: Neuropharmacology; 26(8): 1167-71
Abstract: The effect of varying the ratio of extraction volume to tissue weight (EVR) on the apparent concentration of histamine (HA) in the hypothalamus of the rat was examined. Increasing the weight of tissue (by pooling 1, 2 or 3 hypothalami), in a constant extraction volume, resulted in progressive decreases in apparent concentration of histamine in the hypothalamus. These concentrations were 642, 450 and 282 ng/g, respectively. Morphine (10 mg/kg, i.p.) significantly reduced the concentration of histamine in the hypothalamus. Expressed as percentages of the saline-control values (obtained for the extraction volume to tissue weight of 35.7, 57.2 and 118.4 ml/g), treatment with morphine resulted in 24, 17 and 11% reductions in the concentration of histamine in the hypothalamus, respectively. However, expressed in terms of ng/g, the reductions in histamine induced by morphine were 68, 75 and 69 ng/g, respectively. It is concluded that morphine may consistently affect a single pool of histamine. The possibility that de novo histamine is formed in the homogenate during the extraction process is discussed.
Internal ID Number: 3658120
URI: http://ahro.austin.org.au/austinjspui/handle/1/13000
URL: http://www.ncbi.nlm.nih.gov/pubmed/3658120
Type: Journal Article
Subjects: Animals
Chromatography, Gel
Female
Histamine.analysis
Hypothalamus.analysis.drug effects
Morphine.administration & dosage.pharmacology
Rats
Rats, Inbred Strains
Spectrophotometry
Time Factors
Appears in Collections:Journal articles

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