Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/12978
Title: Contractile activity and reperfusion-induced calcium gain after ischemia in the isolated rat heart.
Authors: Elz, J S;Nayler, W G
Affiliation: Department of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Victoria, Australia.
Issue Date: 1-Jun-1988
Citation: Laboratory Investigation; A Journal of Technical Methods and Pathology; 58(6): 653-9
Abstract: Reperfusion of cardiac muscle after an ischemic episode results in the cells becoming overloaded with Ca2+. Gross ultrastructural changes, including the formation of contraction bands, also occur. The present study investigates the relationship, if any, between contractile activity during reperfusion and Ca2+ gain. Contractile activity was inhibited with 2,3-butanedione monoxime (BDM). Isolated perfused rat hearts were subjected to 30 minutes ischemia before reperfusion in the presence or absence of BDM. BDM (10 MIN) significantly reduced the Ca2+ gained during reperfusion. It also enhanced the ATP and creatine phosphate supplies. Ultrastructural examination of cells from hearts reperfused in the presence of BDM for 30 minutes revealed cells with relaxed myofibrils, some glycogen and intact sarcolemmal membranes, compared with cells from hearts reperfused in the absence of BDM which showed contraction bands, sarcolemmal discontinuities and swollen mitochondria. The 'protection' afforded by BDM did not result in a restoration of the cells to their normal state. Removal of BDM and continued reperfusion with Krebs-Henseleit buffer resulted in a gain in Ca2+ and ultrastructural damage, including contraction band formation. These findings suggest a role for contractile activity in the Ca2+ gain. However, preventing the damage which occurs as a result of contractile activity is not sufficient to restore the cells to their preischemic state. This suggests that the damage caused as a result of contractile activity is secondary to some other primary deleterious event.
Internal ID Number: 3379913
URI: http://ahro.austin.org.au/austinjspui/handle/1/12978
URL: http://www.ncbi.nlm.nih.gov/pubmed/3379913
Type: Journal Article
Subjects: Animals
Calcium.metabolism
Coronary Circulation
Coronary Disease.physiopathology
Diacetyl.pharmacology
Female
Myocardial Contraction
Rats
Rats, Inbred Strains
Appears in Collections:Journal articles

Files in This Item:
There are no files associated with this item.


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.