Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/12974
Title: Hepatic metabolism of vasoactive intestinal polypeptide (VIP) in the rat.
Authors: Brook, C W;Sewell, Richard B;Shulkes, Arthur;Smallwood, R A
Affiliation: Department of Medicine, Austin Hospital, Heidelberg, Australia.
Issue Date: 1-Apr-1988
Citation: Regulatory Peptides; 20(4): 311-22
Abstract: Vasoactive intestinal polypeptide (VIP) is released into the portal circulation by a meal stimulus, but is rapidly cleared from plasma. Although it is known to bind to receptors on liver cells, the role of the liver in the clearance of VIP is not clearly defined. We therefore studied the disappearance of VIP in recirculating and in single pass isolated perfused rat liver (IPRL) preparations. Disappearance of added VIP was rapid in recirculating IPRL experiments with a half life of ca. 30 min. In single-pass steady-state studies in which livers were perfused at 16 ml/min for 30 min, clearance of VIP was complete (16 ml/min) at concentrations of 500 fmol/ml, but clearance fell to 3 and 1 ml/min at perfusate concentrations of 8 and 40 pmol/ml respectively. Further experiments to evaluate whether VIP was disappearing in perfusate itself demonstrated substantial metabolism of VIP in perfusate which had previously been circulated through a liver for 90 min. The products of metabolism were identical to those found in the IPRL. We conclude that VIP is rapidly cleared as it passes through the isolated perfused rat liver model with a significant proportion of clearance attributable to release of a peptidase from the liver into the perfusate.
Internal ID Number: 3368582
URI: http://ahro.austin.org.au/austinjspui/handle/1/12974
URL: http://www.ncbi.nlm.nih.gov/pubmed/3368582
Type: Journal Article
Subjects: Animals
Female
In Vitro Techniques
Liver.metabolism
Metabolic Clearance Rate
Perfusion
Rats
Rats, Inbred Strains
Vasoactive Intestinal Peptide.metabolism
Appears in Collections:Journal articles

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