Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/12875
Title: Inhibition of angiotensin converting enzyme (ACE) in plasma and tissues: studies ex vivo after administration of ACE inhibitors.
Authors: Johnston, Colin I;Mendelsohn, Frederick AO;Cubela, R B;Jackson, B;Kohzuki, M;Fabris, Bruno
Affiliation: University of Melbourne, Department of Medicine, Austin Hospital, Heidelberg, Victoria, Australia.
Issue Date: 1-Dec-1988
Citation: Journal of Hypertension. Supplement : Official Journal of the International Society of Hypertension; 6(3): S17-22
Abstract: Two methods of radio-inhibitor binding to tissue membrane homogenates and in vitro autoradiography have been used for ex vivo studies on the inhibition of tissue angiotensin converting enzyme (ACE) following acute and chronic administration of ACE inhibitors. Tissue ACE is differentially inhibited in time and degree in different tissues of the rat. Plasma and kidney ACE are inhibited completely at low doses whereas lung and aorta are only inhibited by 60-70%, even after very high does of ACE inhibitors. In the brain only those structures outside the blood-brain barrier are inhibited at low doses but at high doses perindopril appears able to cross the blood-brain barrier. Similarly, testicular ACE is not inhibited and appears to be protected by a blood-testis barrier. Preliminary results suggest that after chronic administration there is also a variable pattern of induction and inhibition of ACE in different tissues. By relating the degree of tissue inhibition to physiological responses it may be possible to determine the role of local renin-angiotensin systems in regional haemodynamics and in the hypotensive action of ACE inhibitors. Further, the techniques of radioligand inhibitor binding and in vitro autoradiography can be extended to other important cardiovascular enzymes (renin and kallikrein) when suitable high affinity specific inhibitors become available.
Internal ID Number: 2852234
URI: http://ahro.austin.org.au/austinjspui/handle/1/12875
URL: http://www.ncbi.nlm.nih.gov/pubmed/2852234
Type: Journal Article
Subjects: Angiotensin-Converting Enzyme Inhibitors.administration & dosage.pharmacology
Animals
Aorta.enzymology
Autoradiography
Benzazepines.pharmacology
Brain.enzymology
In Vitro Techniques
Indoles.administration & dosage.pharmacology
Isoquinolines.pharmacology
Kidney.enzymology
Lung.enzymology
Male
Peptidyl-Dipeptidase A.blood.metabolism
Perindopril
Radioligand Assay
Rats
Testis.enzymology
Tetrahydroisoquinolines
Appears in Collections:Journal articles

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