Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/12859
Title: Characterization of angiotensin converting enzyme (ACE) in the testis and assessment of the in vivo effects of the ACE inhibitor perindopril.
Authors: Jackson, B;Cubela, R B;Sakaguchi, K;Johnston, Colin I
Affiliation: University of Melbourne, Department of Medicine, Austin Hospital, Heidelberg, Victoria, Australia.
Issue Date: 1-Jul-1988
Citation: Endocrinology; 123(1): 50-5
Abstract: Angiotensin converting enzyme (ACE) was characterized by radioligand studies utilizing the potent ACE inhibitor 351A, a derivative of lisinopril. Ligand binding characteristics were similar for ACE derived from testis, lung, and kidney, despite known differences in structure between ACe from these sources. This observation suggests that the ACE active enzymatic site is similar in different tissues. The effect of the orally active ACE inhibitor perindopril was studied ex vivo in tissues of the rat after oral gavage. Radioligand bound to tissue ACE was reduced after perindopril treatment, in tissue homogenates of lung and kidney, but not testis. Autoradiographs of radioligand binding to tissue sections obtained ex vivo after oral perindopril showed inhibition of ACE in the aorta, lung, and kidney, but did not reveal any inhibition of ACE in the testis. ACE in small vessels of the testis was inhibited as in the aorta, while at the same time testicular ACE was unaffected. ACE in rat testis appears to have a similar enzymatic binding site to ACE from the lung and kidney. Perindopril inhibited ACE in the lung and kidney but did not affect ACE in the testis, suggesting the drug is limited in testicular penetration by the blood-testis barrier. This may explain the lack of any reports of adverse effects of ACE inhibitors on testicular function.
Internal ID Number: 2838262
URI: http://ahro.austin.org.au/austinjspui/handle/1/12859
DOI: 10.1210/endo-123-1-50
URL: http://www.ncbi.nlm.nih.gov/pubmed/2838262
Type: Journal Article
Subjects: Angiotensin-Converting Enzyme Inhibitors.pharmacology
Animals
Aorta.enzymology
Autoradiography
Indoles.pharmacology
Iodine Radioisotopes
Kidney.enzymology
Kinetics
Liver.enzymology
Male
Peptidyl-Dipeptidase A.metabolism
Perindopril
Rats
Rats, Inbred Strains
Testis.enzymology
Appears in Collections:Journal articles

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