Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/12838
Title: Radioimmunoassay for immunoreactive [des-Leu10]-angiotensin I.
Authors: Johnson, H;Kourtis, S;Waters, J;Drummer, Olaf H
Affiliation: University of Melbourne, Clinical Pharmacology and Therapeutics Unit, Austin Hospital, Heidelberg, Australia.
Issue Date: 6-May-1989
Citation: Peptides; 10(3): 489-92
Abstract: A specific radioimmunoassay for the angiotensin-derived peptide [des-Leu10]-angiotensin I (AI-dL) is described. Antisera obtained from rabbits injected with immunogen prepared by coupling bovine beta-thyroglobulin to the peptide with carbodiimide were specific to this peptide and did not recognise related angiotensin peptides such as AI, AII, AIII, nor did they recognise other peptides such as bradykinin, substance P, bombesin or dynorphin(1-8). Immunoreactive AI-dL was detected for the first time in the plasma of rats and humans following purification by HPLC at concentrations of 78 and 40 pg/ml, respectively. Concentrations of AI-dL are increased following chronic administration of captopril to rats.
Internal ID Number: 2780412
URI: http://ahro.austin.org.au/austinjspui/handle/1/12838
URL: http://www.ncbi.nlm.nih.gov/pubmed/2780412
Type: Journal Article
Subjects: Angiotensin I.analogs & derivatives.blood.isolation & purification
Animals
Chromatography, High Pressure Liquid
Cross Reactions
Humans
Immune Sera
Rabbits.immunology
Radioimmunoassay.methods
Rats
Appears in Collections:Journal articles

Files in This Item:
There are no files associated with this item.


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.