Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/12796
Title: A Versatile Approach for the Site-Specific Modification of Recombinant Antibodies Using a Combination of Enzyme-Mediated Bioconjugation and Click Chemistry.
Authors: Alt, Karen;Paterson, Brett M;Westein, Erik;Rudd, Stacey E;Poniger, Stan S;Jagdale, Shweta;Ardipradja, Katie;Connell, Timothy U;Krippner, Guy Y;Nair, Ashish K N;Wang, Xiaowei;Tochon-Danguy, Henri J;Donnelly, Paul S;Peter, Karlheinz;Hagemeyer, Christoph E
Affiliation: Vascular Biotechnology, Baker IDI, Melbourne (Australia). karen.alt@bakeridi.edu.au.
Atherothrombosis and Vascular Biology, Baker IDI, Melbourne (Australia).
School of Chemistry/Bio21 Institute, University of Melbourne (Australia).
Vascular Biotechnology, Baker IDI, Melbourne (Australia).
Department of Molecular Imaging and Therapy, Austin Health, Melbourne (Australia).
School of Chemistry/Bio21 Institute, University of Melbourne (Australia). pauld@unimelb.edu.au.
Vascular Biotechnology, Baker IDI, Melbourne (Australia). christoph.hagemeyer@bakeridi.edu.au.
Issue Date: 11-May-2015
Citation: Angewandte Chemie (international Ed. in English) 2015; 54(26): 7515-9
Abstract: A unique two-step modular system for site-specific antibody modification and conjugation is reported. The first step of this approach uses enzymatic bioconjugation with the transpeptidase Sortase A for incorporation of strained cyclooctyne functional groups. The second step of this modular approach involves the azide-alkyne cycloaddition click reaction. The versatility of the two-step approach has been exemplified by the selective incorporation of fluorescent dyes and a positron-emitting copper-64 radiotracer for fluorescence and positron-emission tomography imaging of activated platelets, platelet aggregates, and thrombi, respectively. This flexible and versatile approach could be readily adapted to incorporate a large array of tailor-made functional groups using reliable click chemistry whilst preserving the activity of the antibody or other sensitive biological macromolecules.
Internal ID Number: 25962581
URI: http://ahro.austin.org.au/austinjspui/handle/1/12796
DOI: 10.1002/anie.201411507
URL: http://www.ncbi.nlm.nih.gov/pubmed/25962581
Type: Journal Article
Subjects: PET imaging
Sortase A
bioconjugation
click chemistry
fluorescence imaging
Appears in Collections:Journal articles

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