Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/12610
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dc.contributor.authorStirzaker, Clareen
dc.contributor.authorZotenko, Elenaen
dc.contributor.authorSong, Jenny Zen
dc.contributor.authorQu, Wenjiaen
dc.contributor.authorNair, Shalima Sen
dc.contributor.authorLocke, Warwick Jen
dc.contributor.authorStone, Andrewen
dc.contributor.authorArmstong, Nicola Jen
dc.contributor.authorRobinson, Mark Den
dc.contributor.authorDobrovic, Alexanderen
dc.contributor.authorAvery-Kiejda, Kelly Aen
dc.contributor.authorPeters, Kate Men
dc.contributor.authorFrench, Juliet Den
dc.contributor.authorStein, Sandraen
dc.contributor.authorKorbie, Darren Jen
dc.contributor.authorTrau, Matten
dc.contributor.authorForbes, John Fen
dc.contributor.authorScott, Rodney Jen
dc.contributor.authorBrown, Melissa Aen
dc.contributor.authorFrancis, Glenn Den
dc.contributor.authorClark, Susan Jen
dc.date.accessioned2015-05-16T02:19:38Z
dc.date.available2015-05-16T02:19:38Z
dc.date.issued2015-02-02en
dc.identifier.citationNature Communications 2015; 6(): 5899en
dc.identifier.govdoc25641231en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/12610en
dc.description.abstractEpigenetic alterations in the cancer methylome are common in breast cancer and provide novel options for tumour stratification. Here, we perform whole-genome methylation capture sequencing on small amounts of DNA isolated from formalin-fixed, paraffin-embedded tissue from triple-negative breast cancer (TNBC) and matched normal samples. We identify differentially methylated regions (DMRs) enriched with promoters associated with transcription factor binding sites and DNA hypersensitive sites. Importantly, we stratify TNBCs into three distinct methylation clusters associated with better or worse prognosis and identify 17 DMRs that show a strong association with overall survival, including DMRs located in the Wilms tumour 1 (WT1) gene, bi-directional-promoter and antisense WT1-AS. Our data reveal that coordinated hypermethylation can occur in oestrogen receptor-negative disease, and that characterizing the epigenetic framework provides a potential signature to stratify TNBCs. Together, our findings demonstrate the feasibility of profiling the cancer methylome with limited archival tissue to identify regulatory regions associated with cancer.en
dc.language.isoenen
dc.titleMethylome sequencing in triple-negative breast cancer reveals distinct methylation clusters with prognostic value.en
dc.typeJournal Articleen
dc.identifier.journaltitleNature communicationsen
dc.identifier.affiliationTranslational Genomics &Epigenomics Laboratory, Olivia Newton-John Cancer Research Institute, Melbourne, Victoria 3084, Australiaen
dc.identifier.affiliation1] Pathology Queensland, Princess Alexandra Hospital, Brisbane, Queensland 4102, Australia [2] Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Brisbane, Queensland 4072, Australiaen
dc.identifier.affiliation1] School of Biomedical Sciences and Pharmacy, Faculty of Health, University of Newcastle, Newcastle, New South Wales 2308, Australia [2] Division of Molecular Medicine, Hunter Area Pathology Service and the Hunter Medical Research Institute, John Hunter Hospital, Newcastle, New South Wales 2305, Australiaen
dc.identifier.affiliationAustralian Institute for Bioengineering and Nanotechnology, University of Queensland, Brisbane, Queensland 4072, Australiaen
dc.identifier.affiliationPathology Queensland, Princess Alexandra Hospital, Brisbane, Queensland 4102, Australiaen
dc.identifier.affiliation1] School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia [2] Department of Genetics and Computational Biology, QIMR Berghofer Medical Research Institute, Brisbane, Queensland 4029, Australiaen
dc.identifier.affiliationSchool of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australiaen
dc.identifier.affiliationSchool of Biomedical Sciences and Pharmacy, Faculty of Health, University of Newcastle, Newcastle, New South Wales 2308, Australiaen
dc.identifier.affiliation1] Epigenetics Group, Cancer Division, Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia [2] School of Mathematics and Statistics, University of Sydney, Sydney, New South Wales 2006, Australiaen
dc.identifier.affiliationEpigenetics Group, Cancer Division, Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australiaen
dc.identifier.affiliation1] Epigenetics Group, Cancer Division, Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia [2] St Vincent's Clinical School, University of NSW, Sydney, New South Wales 2010, Australiaen
dc.identifier.affiliationSchool of Medicine and Public Health, Faculty of Health and Medicine, University of Newcastle, Newcastle, New South Wales 2305, Australiaen
dc.identifier.affiliation1] School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia [2] Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Brisbane, Queensland 4072, Australiaen
dc.identifier.affiliation1] Epigenetics Group, Cancer Division, Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia [2] Swiss Institute of Bioinformatics and Institute of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.en
dc.identifier.doi10.1038/ncomms6899en
dc.description.pages5899en
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/25641231en
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