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|Title:||Ischaemia causes externalization of endothelin-1 binding sites in rat cardiac membranes.|
|Authors:||Liu, J J;Casley, David J;Nayler, W G|
|Affiliation:||Department of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Victoria, Australia|
|Citation:||Biochemical and Biophysical Research Communications; 164(3): 1220-5|
|Abstract:||Specific, high affinity binding sites for iodinated endothelin-1 ([125I]-ET-1) were identified in crude plasma and light membrane fractions harvested from aerobically perfused and ischaemic rat hearts, to determine whether the ischaemia-induced increase in binding site density (Bmax) involves externalization of the sites. In crude plasma membranes Bmax increased after 60 min ischaemia, from 113.5 +/- 2.15 to 180.6 +/- 4.67 fmol/mg protein (p less than 0.01). In the light membranes, the Bmax fell, from 94.7 +/- 8.70 to 63.80 +/- 6.26 fmol/mg protein (p less than 0.05). Hill coefficients and selectivity of both membrane fractions were unchanged. These results are interpreted as meaning that ischaemia causes externalization of cardiac [125I]-ET-1 binding sites.|
|Internal ID Number:||2556121|
Rats, Inbred Strains
Receptors, Cell Surface.metabolism
|Appears in Collections:||Journal articles|
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