Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/12279
Title: Hepatitis C virus-induced hepatocyte cell death and protection by inhibition of apoptosis.
Authors: Lim, Eu Jin;El Khobar, Korri;Chin, Ruth;Earnest-Silveira, Linda;Angus, Peter W;Bock, C-Thomas;Nachbur, Ueli;Silke, John;Torresi, Joseph
Affiliation: Department of Medicine, Austin Hospital, University of Melbourne, Heidelberg, Victoria, Australia Department of Gastroenterology and Hepatology, Austin Hospital, Heidelberg, Victoria, Australia
Walter and Eliza Hall Institute, Parkville, Victoria, Australia
Department of Medicine, Austin Hospital, University of Melbourne, Heidelberg, Victoria, Australia
Department of Virology, Robert Koch Institute, Berlin, Germany.
Department of Medicine, Austin Hospital, University of Melbourne, Heidelberg, Victoria, Australia Department Infectious Diseases, Austin Hospital, Heidelberg, Victoria, Australia josepht@unimelb.edu.au.
Issue Date: 27-Jun-2014
Citation: The Journal of General Virology 2014; 95(Pt 10): 2204-15
Abstract: Chronic hepatitis C virus (HCV) infection results in progressive liver fibrosis leading to cirrhosis and liver cancer. The mechanism for this remains unclear but hepatocyte apoptosis is thought to play a major role. Hepatocyte apoptosis in human liver tissue was determined by immunohistochemistry for cytokeratin 18 (M30 CytoDEATH) and cleaved poly(ADP-ribose) polymerase (PARP). In vitro studies were performed with replication-defective recombinant adenoviruses expressing HCV proteins (rAdHCV) to study the effects of HCV on cell death in Huh7 cells, primary mouse hepatocytes (PMoHs) and primary human hepatocytes (PHHs). Cell viability and apoptosis were studied using crystal violet assays and Western blots probed for cleaved caspase-3 and cleaved PARP, with and without treatment with the pan-caspase inhibitor Q-VD-OPh and necrostatin-1. Liver tissue of HCV-infected patients expressed elevated levels of apoptotic markers compared with HCV-negative patients. rAdHCV infection reduced cell viability compared with uninfected controls and cells infected with control virus (rAdGFP). Huh7, PMoHs and PHHs infected with rAdHCV showed significantly increased levels of apoptotic markers compared with uninfected controls and rAdGFP-infected cells. In rAdHCV-infected Huh7, treatment with Q-VD-OPh and necrostatin-1 both improved cell viability. Q-VD-Oph also reduced cleaved PARP in rAdHCV-infected Huh7 and PMoHs. Hepatocyte apoptosis is known to be increased in the livers of HCV-infected patients. HCV promoted cell death in primary and immortalized hepatocytes, and this was inhibited by Q-VD-OPh and necrostatin-1. These findings indicate that HCV-induced cell death occurs by both apoptosis and necroptosis, and provide new insights into the mechanisms of HCV-induced liver injury.
Internal ID Number: 24973240
URI: http://ahro.austin.org.au/austinjspui/handle/1/12279
DOI: 10.1099/vir.0.065862-0
URL: http://www.ncbi.nlm.nih.gov/pubmed/24973240
Type: Journal Article
Subjects: Amino Acid Chloromethyl Ketones.metabolism
Animals
Apoptosis
Caspases.analysis
Cell Survival
Enzyme Inhibitors.metabolism
Hepacivirus.physiology
Hepatitis C.pathology
Hepatocytes.drug effects.physiology.virology
Humans
Imidazoles.metabolism
Indoles.metabolism
Mice
Mice, Inbred C57BL
Necrosis
Quinolines.metabolism
Appears in Collections:Journal articles

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