Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/12095
Title: A critical re-assessment of DNA repair gene promoter methylation in non-small cell lung carcinoma.
Authors: Do, Hongdo;Wong, Nicholas C;Murone, Carmel;John, Thomas;Solomon, Benjamin J;Mitchell, Paul L R;Dobrovic, Alexander
Affiliation: Department of Pathology, University of Melbourne, Parkville, Victoria, 3010, Australia
Division of Cancer Medicine, Peter MacCallum Cancer Centre, Melbourne, Victoria, 8006, Australia
Olivia Newton John Cancer Wellness and Research Centre, Austin Health, Heidelberg, Victoria, Australia
Ludwig Institute for Cancer Research, Austin Health, Heidelberg, Victoria, Australia
Olivia Newton-John Cancer Research Institute, Heidelberg, Victoria, Australia
Molecular Pathology Research and Development Laboratory, Department of Pathology, Peter MacCallum Cancer Centre, Melbourne, Victoria, 8006, Australia
Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, Victoria, 3010, Australia
Joint Austin-Ludwig Medical Oncology Department, Olivia Newton John Cancer Wellness and Research Centre, Austin Health, Heidelberg, Victoria, Australia
Issue Date: 26-Feb-2014
Citation: Scientific Reports 2014; 4: 4186
Abstract: DNA repair genes that have been inactivated by promoter methylation offer potential therapeutic targets either by targeting the specific repair deficiency, or by synthetic lethal approaches. This study evaluated promoter methylation status for eight selected DNA repair genes (ATM, BRCA1, ERCC1, MGMT, MLH1, NEIL1, RAD23B and XPC) in 56 non-small cell lung cancer (NSCLC) tumours and 11 lung cell lines using the methylation-sensitive high resolution melting (MS-HRM) methodology. Frequent methylation in NEIL1 (42%) and infrequent methylation in ERCC1 (2%) and RAD23B (2%) are reported for the first time in NSCLC. MGMT methylation was detected in 13% of the NSCLCs. Contrary to previous studies, methylation was not detected in ATM, BRCA1, MLH1 and XPC. Data from The Cancer Genome Atlas (TCGA) was consistent with these findings. The study emphasises the importance of using appropriate methodology for accurate assessment of promoter methylation.
Internal ID Number: 24569633
URI: http://ahro.austin.org.au/austinjspui/handle/1/12095
DOI: 10.1038/srep04186
URL: http://www.ncbi.nlm.nih.gov/pubmed/24569633
Type: Journal Article
Subjects: Carcinoma, Non-Small-Cell Lung.genetics
DNA Methylation.genetics
DNA Repair
DNA, Neoplasm.genetics
Humans
Lung Neoplasms.genetics
Neoplasm Proteins.genetics
Promoter Regions, Genetic.genetics
Tumor Cells, Cultured
Appears in Collections:Journal articles

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