Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/12074
Title: Nicotine-induced calcium overload during postischemic reperfusion.
Authors: Panagiotopoulos, Sianna;Nayler, W G
Affiliation: Department of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Victoria, Australia.
Issue Date: 1-Dec-1987
Citation: Journal of Cardiovascular Pharmacology; 10(6): 683-91
Abstract: The excessive gain in Ca2+ that occurs when hearts are reperfused after prolonged periods of ischemia contributes to cell death and tissue necrosis. The following experiments were undertaken to determine whether nicotine, in a concentration equivalent to the peak concentration present in plasma after cigarette smoke inhalation, alters reperfusion-induced Ca2+ gain in isolated rat hearts. Nicotine (0.15 microgram/ml) failed to increase tissue Ca2+ during aerobic perfusion but increased Ca2+ gain during reperfusion after 30 (p less than 0.02) or 60 (p less than 0.02) min of normothermic ischemia. The increase of Ca2+ gain was independent of a nicotine-induced release of norepinephrine (NE) or an altered "reflow area", heart rate, force of contraction, or end-diastolic resting tension. Pretreatment for 3 days with anipamil (20 mg/kg), a long-acting calcium channel blocker, attenuated the reperfusion-induced Ca2+ gain after 30 min of global ischemia, and reduced (p less than 0.001) the nicotine-induced exacerbation of that gain, without altering tissue ATP or creatine phosphate (CP). Verapamil (1 X 10(-6) M) reduced (p less than 0.02) the nicotine-induced exacerbation of Ca2+ gain caused by reperfusion after 30 min of ischemia.
Internal ID Number: 2450239
URI: http://ahro.austin.org.au/austinjspui/handle/1/12074
ORCID: 0000-0002-0845-0001
URL: http://www.ncbi.nlm.nih.gov/pubmed/2450239
Type: Journal Article
Subjects: Adenosine Triphosphate.analysis
Animals
Calcium.metabolism
Calcium Channel Blockers.pharmacology
Coronary Circulation.drug effects
Coronary Disease.metabolism.physiopathology
Female
Heart Rate.drug effects
In Vitro Techniques
Nicotine.toxicity
Norepinephrine.metabolism
Perfusion
Phosphocreatine.analysis
Propylamines.pharmacology
Rats
Rats, Inbred Strains
Appears in Collections:Journal articles

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