Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/12047
Title: Angiotensin converting enzyme induction by cyclic AMP and analogues in cultured endothelial cells.
Authors: Lloyd, C J;Cary, D A;Mendelsohn, Frederick AO
Affiliation: University of Melbourne, Department of Medicine, Austin Hospital, Victoria, Australia.
Issue Date: 1-Aug-1987
Citation: Molecular and Cellular Endocrinology; 52(3): 219-25
Abstract: The role of cyclic AMP in regulating the production of angiotensin converting enzyme (ACE) was investigated using cultured bovine aortic endothelial cells. Addition of dibutyryl cAMP [Bu)2cAMP) at 100 microM increased the ACE activity to 126% of control (P less than 0.005). This effect was blocked by either actinomycin D (0.1 microgram/ml) or cycloheximide (1.7 microM) indicating that RNA as well as protein synthesis was required for induction of the enzyme. After addition of (Bu)2cAMP, a lag period of 8 h was observed before increased ACE activity was detected. The stable analogues, 8-bromo cAMP (100 microM) and N6-monobutyryl cAMP (100 microM) also increased ACE activity but cAMP (100 microM) and O2-monobutyryl cAMP (100 microM) had no effect, in keeping with their susceptibility to phosphodiesterase in this system. Sodium butyrate (100 microM) was also inactive. The effect of (Bu)2cAMP on ACE was still observed in the presence of a maximal dose of dexamethasone, indicating that (Bu)2cAMP stimulates by mechanism(s) independent of the previously observed action of glucocorticoids on these cells. The phosphodiesterase inhibitor IBMX caused a dose-related increase in ACE activity with a threshold at 30 microM (P less than 0.05) and produced a 4-fold increase above control at 1 mM IBMX.
Internal ID Number: 2443404
URI: http://ahro.austin.org.au/austinjspui/handle/1/12047
URL: http://www.ncbi.nlm.nih.gov/pubmed/2443404
Type: Journal Article
Subjects: 1-Methyl-3-isobutylxanthine.pharmacology
Animals
Cells, Cultured
Cyclic AMP.analogs & derivatives.pharmacology
Cycloheximide.pharmacology
Dactinomycin.pharmacology
Dexamethasone.pharmacology
Endothelium, Vascular.drug effects.enzymology
Enzyme Activation.drug effects
Peptidyl-Dipeptidase A.biosynthesis
Appears in Collections:Journal articles

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