Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/12022
Title: Fully automated synthesis and coupling of [(18) F]FBEM to glutathione using the iPHASE FlexLab module.
Authors: Ackermann, Uwe;Plougastel, Lucie;Wichmann, Christian;Goh, Yit Wooi;Yeoh, Shinn Dee;Poniger, Stan S;Tochon-Danguy, Henri J;Scott, Andrew M
Affiliation: Department of Nuclear Medicine and Centre for PET, Austin Health, Heidelberg, Melbourne, Australia; The University of Melbourne, Parkville, Melbourne, Australia; Ludwig Institute for Cancer Research, Melbourne Branch, Melbourne, Australia.
Issue Date: 7-Jan-2014
Citation: Journal of Labelled Compounds & Radiopharmaceuticals 2014; 57(2): 115-20
Abstract: Site-specific radiolabelling of peptides or antibodies using [(18) F]FBEM is often preferred over non-site-specific radiolabelling with [(18) F]SFB because it does not affect the affinity of the antibody to its target. Unfortunately, the synthesis of [(18) F]FBEM and its conjugation to thiol containing macromolecules requires some manual intervention, which leads to radiation exposure of the radiochemist. In this publication, we report on the complete automation of [(18) F]FBEM production and its subsequent conjugation to glutathione using a slightly modified iPHASE FlexLab module. [(18) F]FBEM was produced in 1.185 ± 0.168 GBq (15-20%; n = 10; 0.75 ± 0.106 GBq non-decay corrected) with a specific activity of 57 ± 10 GBq/µmol. Radiochemical purity was 97 ± 1% and the synthesis time including HPLC purification and reformulation was 70 min. After evaporation to dryness, [(18) F]FBEM was conjugated to glutathione in PBS buffer pH 7.4 in quantitative yields. This fully automated method does not require any manual intervention and therefore reduces the radiation exposure to the operator.
Internal ID Number: 24395455
URI: http://ahro.austin.org.au/austinjspui/handle/1/12022
DOI: 10.1002/jlcr.3175
URL: http://www.ncbi.nlm.nih.gov/pubmed/24395455
Type: Journal Article
Subjects: FBEM
PET
peptide labelling
radiolabelled synthons
Automation, Laboratory
Glutathione.chemical synthesis
Isotope Labeling.methods
Maleimides.chemical synthesis
Radiopharmaceuticals.chemical synthesis
Appears in Collections:Journal articles

Files in This Item:
There are no files associated with this item.


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.