Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/12018
Title: Hypothermia protects human neurons.
Authors: Antonic, Ana;Dottori, Mirella;Leung, Jessie;Sidon, Kate;Batchelor, Peter Egerton;Wilson, William;Macleod, Malcolm R;Howells, David William
Affiliation: Florey Institute of Neuroscience and Mental Health, Heidelberg, Vic, Australia; Department of Medicine, University of Melbourne, Heidelberg, Vic, Australia.
Issue Date: 3-Jan-2014
Citation: International Journal of Stroke : Official Journal of the International Stroke Society 2014; 9(5): 544-52
Abstract: Hypothermia provides neuroprotection after cardiac arrest, hypoxic-ischemic encephalopathy, and in animal models of ischemic stroke. However, as drug development for stroke has been beset by translational failure, we sought additional evidence that hypothermia protects human neurons against ischemic injury.Human embryonic stem cells were cultured and differentiated to provide a source of neurons expressing β III tubulin, microtubule-associated protein 2, and the Neuronal Nuclei antigen. Oxygen deprivation, oxygen-glucose deprivation, and H2 O2 -induced oxidative stress were used to induce relevant injury.Hypothermia to 33°C protected these human neurons against H2 O2 -induced oxidative stress reducing lactate dehydrogenase release and Terminal deoxynucleotidyl transferase dUTP nick end labeling-staining by 53% (P ≤ 0·0001; 95% confidence interval 34·8-71·04) and 42% (P ≤ 0·0001; 95% confidence interval 27·5-56·6), respectively, after 24 h in culture. Hypothermia provided similar protection against oxygen-glucose deprivation (42%, P ≤ 0·001, 95% confidence interval 18·3-71·3 and 26%, P ≤ 0·001; 95% confidence interval 12·4-52·2, respectively) but provided no protection against oxygen deprivation alone. Protection (21%) persisted against H2 O2 -induced oxidative stress even when hypothermia was initiated six-hours after onset of injury (P ≤ 0·05; 95% confidence interval 0·57-43·1).We conclude that hypothermia protects stem cell-derived human neurons against insults relevant to stroke over a clinically relevant time frame. Protection against H2 O2 -induced injury and combined oxygen and glucose deprivation but not against oxygen deprivation alone suggests an interaction in which protection benefits from reduction in available glucose under some but not all circumstances.
Internal ID Number: 24393199
URI: http://ahro.austin.org.au/austinjspui/handle/1/12018
DOI: 10.1111/ijs.12224
URL: http://www.ncbi.nlm.nih.gov/pubmed/24393199
Type: Journal Article
Subjects: brain
hypothermia
ischemic stroke
neuroprotection
stem cells
treatment
Apoptosis.physiology
Cell Death.physiology
Cell Hypoxia
Cells, Cultured
Embryonic Stem Cells
Glucose.deficiency
Humans
Hydrogen Peroxide.toxicity
Hypothermia.physiopathology
Hypothermia, Induced
Lactate Dehydrogenases.metabolism
Neurons.physiology
Oxidative Stress.physiology
Oxygen.metabolism
Time Factors
Appears in Collections:Journal articles

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