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|Title:||The increased sensitivity of human cytomegalovirus (HCMV) PCR quantitation in whole blood affects reproductive rate (Ro) measurement.|
|Authors:||Gurtler, Volker;Mayall, Barrie C;Wang, Jenny;Ghaly-Derias, Shahbano|
|Affiliation:||School of Applied Sciences, Building 223, Level 1, Bundoora Campus RMIT University, PO Box 71, Bundoora 3083, Australia|
Department of Pathology, Austin Hospital, Studley Road, Heidelberg 3084, Australia
Electronic address: firstname.lastname@example.org.
|Citation:||Journal of Virological Methods 2013; 196(): 179-84|
|Abstract:||In order to determine the effect of the increase in sensitivity of HCMV detection in whole blood compared to plasma on reproductive rate (Ro) measurement, an optimized human cytomegalovirus (HCMV) quantitative PCR assay was developed. The results presented in this study are summarized by the following three methodological improvements: (i) at values below the limit of quantitation (LOQ) of 60copies/ml, determination of HCMV load was more sensitive with whole blood than plasma, (ii) for the determination of viral load, whole blood was more sensitive than plasma below 1000copies/ml but little difference was observed above 1000copies/ml and (iii) the measurement of "Reproductive Rate" can be affected by imprecise measurement of HCMV viral load in either plasma or whole blood compartments depending on whether samples were taken from patients on antiviral treatment or from patients where HCMV load was rising. Taken together this study provides methodological improvements suggesting that below HCMV viral load levels of 1000copies/ml (1640IU/ml) both plasma and whole blood should be tested.|
|Internal ID Number:||24269795|
Quantitative real-time PCR
limit of quantitation
lower limit of detection
single nucleotide polymorphism
Basic Reproduction Number
Cytomegalovirus.growth & development.isolation & purification
Real-Time Polymerase Chain Reaction.methods
Sensitivity and Specificity
|Appears in Collections:||Journal articles|
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