Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/11847
Title: An in vivo mouse model of intraosseous spinal cancer causing evolving paraplegia.
Authors: Cossigny, Davina A F;Mouhtouris, Effie;Dushyanthen, Sathana;Gonzalvo, Augusto;Quan, Gerald M Y
Affiliation: Spinal Biology Research Laboratory, Department of Spinal Surgery, University of Melbourne Department of Surgery, Austin Health, 145 Studley Road, PO Box 5555, Heidelberg, VIC, 3084, Australia.
Issue Date: 18-Aug-2013
Citation: Journal of Neuro-oncology 2013; 115(2): 189-96
Abstract: The spine is the commonest site of skeletal metastatic disease and uncontrolled growth of cancer in the spine will inevitably cause pain and neurologic compromise. Improved understanding of the pathobiology behind this devastating condition is urgently needed. For this reason, the aim of this study was to establish a clinically relevant, animal model of spinal cancer. A percutaneous orthotopic injection of human breast (MDA-MB-231) or human prostate (PC-3) cancer cells was administered into the upper lumbar spine of nude mice (n = 6). Animals were monitored twice daily for general welfare, gait asymmetry or disturbance, and hindlimb weakness. After sacrifice, plain radiographs, micro-CT imaging and histological analysis of the spines were performed on each mouse. All mice recovered fully from the inoculation procedure and displayed normal gait and behaviour patterns for at least 3 weeks post-inoculation. Subsequently, between 3 and 5 weeks post-inoculation, each mouse developed evolving paralysis in their hindlimbs over 48-72 h. All followed the same pattern of decline following onset of neurological dysfunction; from gait asymmetry and unilateral hindlimb weakness, to complete unilateral hindlimb paralysis and finally to complete bilateral hindlimb paralysis. Plain radiographs, micro-CT scanning and histological analysis confirmed local tumour growth and destruction of the spine in all six mice. An in vivo mouse model of human intraosseous spinal cancer has been established forming cancers that grow within the spine and cause epidural spinal cord compression, resulting in a reproducible, evolving neurological deficit and paralysis that closely resembles the human condition.
Internal ID Number: 23955595
URI: http://ahro.austin.org.au/austinjspui/handle/1/11847
DOI: 10.1007/s11060-013-1226-z
URL: http://www.ncbi.nlm.nih.gov/pubmed/23955595
Type: Journal Article
Subjects: Animals
Breast Neoplasms.pathology
Disease Models, Animal
Female
Humans
Injections, Spinal
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Paraplegia.etiology.pathology.radiography
Prostatic Neoplasms.pathology
Spinal Neoplasms.pathology.radiography
Tumor Cells, Cultured
Appears in Collections:Journal articles

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