Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/11785
Title: The C-terminal flanking peptide of progastrin induces gastric cell apoptosis and stimulates colonic cell division in vivo.
Authors: Marshall, Kathryn M;Patel, Oneel;Bramante, Gianni;Laval, Marie;Yim, Mildred;Baldwin, Graham S;Shulkes, Arthur
Affiliation: Department of Surgery, University of Melbourne, Austin Health, Lance Townsend Building, Lvl 8, Studley Road, Heidelberg, Victoria, Australia 3084. kma@unimelb.edu.au
Issue Date: 3-Jun-2013
Citation: Peptides 2013; 46(): 83-93
Abstract: Progastrin (PG) is processed into a number of smaller peptides including amidated gastrin (Gamide), non-amidated glycine-extended gastrin (Ggly) and the C-terminal flanking peptide (CTFP). Several groups have reported that PG, Gamide and Ggly are biologically active in vitro and in vivo, and are involved in the development of gastrointestinal cancers. CTFP is bioactive in vitro but little is known of its effects in vivo. This study investigated the bioactivity of CTFP in vivo in normal tissues using gastrin deficient (GASKO) mice and in two mouse models of cancer (SCID mice bearing xenograft tumors expressing normal or knocked-down levels of gastrin and a mouse model of hepatic metastasis). As with Ggly, CTFP treatment stimulated colonic proliferation in GASKO mice compared to control. CTFP also significantly increased apoptosis in the gastric mucosa of male GASKO mice. CTFP did not appear to effect xenograft growth or the incidence of liver metastases. This is the first demonstration that CTFP has specific biological activity in vivo in the colon and stomach.
Internal ID Number: 23742999
URI: http://ahro.austin.org.au/austinjspui/handle/1/11785
DOI: 10.1016/j.peptides.2013.05.009
URL: http://www.ncbi.nlm.nih.gov/pubmed/23742999
Type: Journal Article
Subjects: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
AS
C-terminal flanking peptide
CRC
CTFP
Cancer
Ci
DMEM
Dulbecco's modified Eagle's medium
ECL
FBS
GASKO
Gamide
Ggly
Gray
Gy
HEPES
Mouse
PBS
PG
Progastrin
RPMI
Roswell Park Memorial Institute medium
SEM
VO
amidated gastrin
antisense
curie
enterochromaffin-like
fetal bovine serum
gastrin knockout
glycine extended gastrin
phosphate buffered saline
progastrin
standard error of the mean
vector only
Animals
Apoptosis
Cell Division.physiology
Cell Line, Tumor
Cell Movement
Cell Proliferation
Female
Gastric Mucosa.metabolism
Gastrins.deficiency.genetics.metabolism
Heterografts
Humans
Liver Neoplasms.metabolism.secondary
Male
Mice
Mice, Inbred CBA
Mice, Knockout
Mice, SCID
Neoplasm Invasiveness
Neoplasm Transplantation
Peptide Fragments.metabolism
Protein Precursors.metabolism
Appears in Collections:Journal articles

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