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https://ahro.austin.org.au/austinjspui/handle/1/11756
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DC Field | Value | Language |
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dc.contributor.author | Lee, D | - |
dc.contributor.author | Desmond, Michael J | - |
dc.contributor.author | Fraser, S A | - |
dc.contributor.author | Katerelos, M | - |
dc.contributor.author | Gleich, Kurt | - |
dc.contributor.author | Berkovic, Samuel F | - |
dc.contributor.author | Power, David Anthony | - |
dc.date.accessioned | 2015-05-16T01:23:00Z | |
dc.date.available | 2015-05-16T01:23:00Z | |
dc.date.issued | 2013-04-26 | - |
dc.identifier.citation | Nephron. Experimental Nephrology 2013; 122(3-4): 103-13 | en_US |
dc.identifier.other | PUBMED | en |
dc.identifier.uri | https://ahro.austin.org.au/austinjspui/handle/1/11756 | en |
dc.description.abstract | Renin processing and storage is believed to occur in lysosome-like structures in the afferent arteriole. SCARB2/Limp-2 is a transmembrane lysosomal protein responsible for the intracellular trafficking of β-glucocerebrosidase. This study aimed to confirm the expression of SCARB2/Limp-2 in renin secretory granules, and explore its role in renin processing and secretion.Co-localisation studies of (pro)renin with lysosomal membrane proteins, SCARB2/Limp-2, LAMP-1 and LAMP-2, were performed in mouse and human kidney sections. Intrarenal expression and secretion of (pro)renin in wild-type (WT) and Limp-2(-/-) mice were compared with and without stimulation.SCARB2/Limp-2, LAMP-1 and LAMP-2 co-localised with (pro)- renin in mouse and human kidney. Plasma renin concentration was increased in Limp-2(-/-) mice when compared to WT littermates. No change in (pro)renin expression, however, was observed in Limp-2(-/-) mouse kidney cortex by immunofluorescence microscopy, Western blotting, quantitative RT-PCR or the ultrastructural appearance of renin secretory granules. Acute stimulation of renin release by isoprenaline or hydralazine was similar in WT and Limp-2(-/-) mice. Following chronic salt restriction, however, immunofluorescence microscopy showed less (pro)renin expressed in Limp-2(-/-) compared with WT mouse kidneys, and there was significantly less prorenin but not renin by Western blotting in Limp-2(-/-) mouse kidney cortex, despite no difference in circulating renin levels.Renin secretory granules possess integral lysosomal proteins, confirming that they are indeed modified lysosomes. Limp-2 deficiency leads to a minor increase in circulating renin. Limp-2, however, is not required for acute or chronic stimulation of renin release. | en_US |
dc.language.iso | en | en |
dc.subject.other | Animals | en |
dc.subject.other | Antigens, CD36.biosynthesis | en |
dc.subject.other | Arterioles.metabolism | en |
dc.subject.other | Female | en |
dc.subject.other | Humans | en |
dc.subject.other | Kidney.blood supply | en |
dc.subject.other | Lysosomal-Associated Membrane Protein 2 | en |
dc.subject.other | Lysosome-Associated Membrane Glycoproteins.biosynthesis | en |
dc.subject.other | Lysosomes.metabolism | en |
dc.subject.other | Male | en |
dc.subject.other | Mice | en |
dc.subject.other | Rats | en |
dc.subject.other | Receptors, Scavenger.biosynthesis | en |
dc.subject.other | Renin.secretion | en |
dc.subject.other | Secretory Vesicles.metabolism | en |
dc.title | Expression of the transmembrane lysosomal protein SCARB2/Limp-2 in renin secretory granules controls renin release. | en_US |
dc.type | Journal Article | en_US |
dc.identifier.journaltitle | Nephron. Experimental Nephrology | en_US |
dc.identifier.affiliation | Nephrology | en_US |
dc.identifier.doi | 10.1159/000350737 | en_US |
dc.description.pages | 103-13 | en |
dc.relation.url | https://pubmed.ncbi.nlm.nih.gov/23635510 | en |
dc.type.content | Text | en_US |
dc.type.austin | Journal Article | en |
local.name.researcher | Berkovic, Samuel F | |
item.openairetype | Journal Article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | none | - |
item.fulltext | No Fulltext | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
crisitem.author.dept | Nephrology | - |
crisitem.author.dept | Institute for Breathing and Sleep | - |
crisitem.author.dept | Epilepsy Research Centre | - |
crisitem.author.dept | Neurology | - |
Appears in Collections: | Journal articles |
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