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Title: A novel population of α-smooth muscle actin-positive cells activated in a rat model of stroke: an analysis of the spatio-temporal distribution in response to ischemia.
Authors: Sharma, Varun;Ling, Tina W;Rewell, Sarah S;Hare, David L;Howells, David William;Kourakis, Angela;Wookey, Peter J
Affiliation: Cardiology Department, Austin Health, Heidelberg, Victoria, Australia
Issue Date: 18-Jul-2012
Citation: Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism 2012; 32(11): 2055-65
Abstract: In a rat model of stroke, the spatio-temporal distribution of α-smooth muscle actin-positive, (αSMA+) cells was investigated in the infarcted hemisphere (ipsilateral) and compared with the contralateral hemisphere. At day 3 postischemia, αSMA+ cells were concentrated in two main loci within the ipsilateral hemisphere (Area A) in the medial corpus callosum and (Area B) midway through the striatum adjacent to the lateral ventricle. By day 7 and further by day 14, fewer αSMA+ cells remained in Areas A and B but a steady increase in the peri-infarct was observed. αSMA+ cells also expressed glial acidic fibrillary protein [GFAP: αSMA+/GFAP+ (29%); αSMA+/GFAP- (71%) phenotypes] and feline leukemia virus C receptor 2 (FLVCR2), but not ED1(microglia) and established markers of pericytes normally located in vascular wall. αSMA+ cells were also located close to the subventricular zones (SVZ) adjacent to Areas A and B. In conclusion, αSMA+ cells have been identified in a spatial and temporal sequence from the SVZ, at intermediate loci and in the vicinity of the peri-infarct. It is hypothesized that novel populations of αSMA+ precursors of pericytes are born on the SVZ, migrate into the peri-infarct region and are incorporated into new vessels of the peri-infarct regions.
Internal ID Number: 22805872
DOI: 10.1038/jcbfm.2012.107
Type: Journal Article
Subjects: Actins.metabolism
Biological Markers
Brain Ischemia.pathology
Fluorescent Antibody Technique
Functional Laterality.physiology
Infarction, Middle Cerebral Artery.pathology
Ki-67 Antigen.metabolism
Microscopy, Confocal
Myocytes, Smooth Muscle.metabolism.pathology.ultrastructure
Paraffin Embedding
Rats, Inbred SHR
Appears in Collections:Journal articles

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