Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/11484
Title: Studies on carbohydrate xenoantigens.
Authors: Christiansen, Dale;Mouhtouris, Effie;Ramsland, Paul A;Sandrin, Mauro S
Affiliation: Department of Surgery (Austin Health), University of Melbourne, Heidelberg, VIC, Australia.
Issue Date: 2012
Citation: Methods in Molecular Biology (clifton, N.j.); 885(): 47-56
Abstract: Naturally occurring and elicited anti-carbohydrate antibodies play a major role in immune responses to xenografts. The original obstacles associated with the Gal antigen have been largely resolved by the generation of knockout pigs. In contrast, much less is known about the nature and role of non-Gal carbohydrate antigens and the antibodies recognizing these. These antibodies can be identified and characterized by enzyme-linked immunosorbent assay. Furthermore, the biological significance of the non-Gal antigen(s) can be determined by expression of the relevant glycosyltransferase(s) by transfection and analyzed by antibody and/or lectin binding.
Internal ID Number: 22565989
URI: http://ahro.austin.org.au/austinjspui/handle/1/11484
DOI: 10.1007/978-1-61779-845-0_4
URL: http://www.ncbi.nlm.nih.gov/pubmed/22565989
Type: Journal Article
Subjects: Animals
Antigens, Heterophile.chemistry.immunology
Carbohydrates.chemistry.immunology
Cell Line
Flow Cytometry
Galactosyltransferases.chemistry.genetics.metabolism
Gene Expression
Humans
Immunohistochemistry
Lectins.metabolism
Protein Binding
Staining and Labeling
Swine
Transfection
Transplantation, Heterologous.immunology
Appears in Collections:Journal articles

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