Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/11421
Title: The outcome of renal ischemia-reperfusion injury is unchanged in AMPK-β1 deficient mice.
Authors: Mount, Peter F;Gleich, Kurt;Tam, Shanna;Fraser, Scott A;Choy, Suet-Wan;Dwyer, Karen M;Lu, Bo;Denderen, Bryce Van;Fingerle-Rowson, Günter;Bucala, Richard;Kemp, Bruce E;Power, David Anthony
Affiliation: Department of Nephrology, Austin Health, Melbourne, Victoria, Australia. Peter.Mount@austin.org.au
Issue Date: 9-Jan-2012
Citation: Plos One 2012; 7(1): e29887
Abstract: Activation of the master energy-regulator AMP-activated protein kinase (AMPK) in the heart reduces the severity of ischemia-reperfusion injury (IRI) but the role of AMPK in renal IRI is not known. The aim of this study was to determine whether activation of AMPK by acute renal ischemia influences the severity of renal IRI.AMPK expression and activation and the severity of renal IRI was studied in mice lacking the AMPK β1 subunit and compared to wild type (WT) mice.Basal expression of activated AMPK, phosphorylayed at αThr¹⁷², was markedly reduced by 96% in AMPK-β1⁻/⁻ mice. Acute renal ischaemia caused a 3.2-fold increase in α1-AMPK activity and a 2.5-fold increase in α2-AMPK activity (P<0.001) that was associated with an increase in AMPK phosphorylation of the AMPK-α subunit at Thr¹⁷² and Ser⁴⁸⁵, and increased inhibitory phosphorylation of the AMPK substrate acetyl-CoA carboxylase. After acute renal ischemia AMPK activity was reduced by 66% in AMPK-β1⁻/⁻ mice compared with WT. There was no difference, however, in the severity of renal IRI at 24-hours between AMPK-β1⁻/⁻ and WT mice, as measured by serum urea and creatinine and histological injury score. In the heart, macrophage migration inhibitory factor (MIF) released during IRI contributes to AMPK activation and protects from injury. In the kidney, however, no difference in AMPK activation by acute ischemia was observed between MIF⁻/⁻ and WT mice. Compared with the heart, expression of the MIF receptor CD74 was found to be reduced in the kidney.The failure of AMPK activation to influence the outcome of IRI in the kidney contrasts with what is reported in the heart. This difference might be due to a lack of effect of MIF on AMPK activation and lower CD74 expression in the kidney.
Internal ID Number: 22253816
URI: http://ahro.austin.org.au/austinjspui/handle/1/11421
DOI: 10.1371/journal.pone.0029887
URL: http://www.ncbi.nlm.nih.gov/pubmed/22253816
Type: Journal Article
Subjects: AMP-Activated Protein Kinases.deficiency.metabolism
Animals
Creatinine.blood
Enzyme Activation
Kidney.blood supply.enzymology.pathology
Macrophage Migration-Inhibitory Factors.deficiency.metabolism
Mice
Phosphorylation
Reperfusion Injury.blood.enzymology.pathology
Urea.blood
Appears in Collections:Journal articles

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