Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/11368
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dc.contributor.authorThurlow, P Jen
dc.contributor.authorKenneally, D Aen
dc.contributor.authorConnellan, J Men
dc.date.accessioned2015-05-16T00:57:30Z
dc.date.available2015-05-16T00:57:30Z
dc.date.issued1990-08-01en
dc.identifier.citationBritish Journal of Haematology; 75(4): 549-56en
dc.identifier.govdoc2207006en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/11368en
dc.description.abstractA monoclonal antibody (anti-Fn2) was prepared which was reactive with both plasma fibronectin and fibronectin located within the platelet alpha granule. Immunoblotting analysis, on thermolysin digestion fragments of fibronectin, identified two immunoreactive fragments of Mr 145 kDa and 155 kDa which are known to contain a cell and DNA binding region. Anti-Fn2 was found to inhibit binding of fibronectin to platelets and DNA. Functional platelet studies, measuring platelet aggregation and 14C-serotonin release in washed platelet systems, demonstrated the ability of anti-Fn2 to totally inhibit low dose thrombin and low-dose collagen induced platelet aggregation and serotonin release. Anti-Fn2 partially inhibited platelet aggregation induced by ADP (10 microM) and arachidonic acid, but had no effect on platelet aggregation induced by high-dose thrombin or by the calcium ionophore A23187. These studies indicate that fibronectin participates in platelet aggregation and release induced by a range of agonists and suggest that it has a more important involvement in platelet function than previously described.en
dc.language.isoenen
dc.subject.otherAntibodies, Monoclonal.diagnostic useen
dc.subject.otherBinding, Competitiveen
dc.subject.otherBlood Platelets.metabolismen
dc.subject.otherFibronectins.metabolism.physiologyen
dc.subject.otherHumansen
dc.subject.otherImmunoblottingen
dc.subject.otherMacromolecular Substancesen
dc.subject.otherPlatelet Aggregation.physiologyen
dc.subject.otherSerotonin.metabolismen
dc.subject.otherThrombin.pharmacologyen
dc.titleThe role of fibronectin in platelet aggregation.en
dc.typeJournal Articleen
dc.identifier.journaltitleBritish journal of haematologyen
dc.identifier.affiliationDepartment of Haematology, Austin Hospital, Heidelberg, Victoria, Australiaen
dc.description.pages549-56en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/2207006en
dc.type.austinJournal Articleen
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
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