Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/11226
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dc.contributor.authorFifis, Theodoraen
dc.contributor.authorMalcontenti-Wilson, Caterinaen
dc.contributor.authorAmijoyo, Jasonen
dc.contributor.authorAnggono, Beatriceen
dc.contributor.authorMuralidharan, Vijayaragavanen
dc.contributor.authorNikfarjam, Mehrdaden
dc.contributor.authorChristophi, Christopheren
dc.date.accessioned2015-05-16T00:48:53Z
dc.date.available2015-05-16T00:48:53Z
dc.date.issued2011-03-02en
dc.identifier.citationHpb : the Official Journal of the International Hepato Pancreato Biliary Association 2011; 13(4): 246-55en
dc.identifier.govdoc21418130en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/11226en
dc.description.abstractThis study examines changes in the expression of growth factors following thermal ablation (TA) of selected colorectal cancer (CRC) liver metastases.Using mice with established CRC liver metastases, two tumours in each animal were thermally ablated. Liver and tumour tissues were collected at various time-points (days 0, 1, 2, 3, 5 and 7) following TA treatment from the ablation site and from sites distant from ablated tumour. Changes in growth factor expression (epidermal growth factor [EGF], vascular endothelial growth factor [VEGF], hepatocyte growth factor [HGF] and transforming growth factor-β[TGF-β]) in comparison with baseline levels (non-ablated) were assessed by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry.Baseline TGF-β and VEGF levels in the liver parenchyma of tumour-bearing mice were significantly higher than levels in naive liver parenchyma. Levels of VEGF and HGF decreased after TA treatment in all tissues. Levels of EGF decreased in ablated and distant tumour tissues, but displayed a tendency to increase in liver tissue. Levels of TGF-β also decreased during the first 2 days following TA, but later increased in liver and tumour tissues distant from the ablation site to a level that reached significance in tumour tissue at day 7 (P < 0.001). Decreases in growth factor levels were also observed in animals that underwent laparotomy without TA treatment, which indicates that these decreases were caused by the experimental procedure.Tumour induces upregulation of TGF-β and VEGF in liver parenchyma. Growth factors decreased after TA, but this appears to be the result of the experimental procedure rather than the TA itself. However, TA resulted in increased levels of TGF-β, which may contribute to tumour recurrence.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherCell Line, Tumoren
dc.subject.otherColorectal Neoplasms.pathologyen
dc.subject.otherEnzyme-Linked Immunosorbent Assayen
dc.subject.otherEpidermal Growth Factor.metabolismen
dc.subject.otherHepatocyte Growth Factor.metabolismen
dc.subject.otherImmunohistochemistryen
dc.subject.otherIntercellular Signaling Peptides and Proteins.metabolismen
dc.subject.otherLaser Therapyen
dc.subject.otherLiver Neoplasms.metabolism.secondary.surgeryen
dc.subject.otherMaleen
dc.subject.otherMiceen
dc.subject.otherMice, Inbred CBAen
dc.subject.otherTime Factorsen
dc.subject.otherTransforming Growth Factor beta.metabolismen
dc.subject.otherVascular Endothelial Growth Factor A.metabolismen
dc.titleChanges in growth factor levels after thermal ablation in a murine model of colorectal liver metastases.en
dc.typeJournal Articleen
dc.identifier.journaltitleHPB : the official journal of the International Hepato Pancreato Biliary Associationen
dc.identifier.affiliationDepartment of Surgery, University of Melbourne, Austin Hospital, Heidelberg, Vic, Australiaen
dc.identifier.doi10.1111/j.1477-2574.2010.00278.xen
dc.description.pages246-55en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/21418130en
dc.type.austinJournal Articleen
local.name.researcherChristophi, Christopher
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
crisitem.author.deptSurgery (University of Melbourne)-
crisitem.author.deptHepatopancreatobiliary Surgery-
crisitem.author.deptSurgery-
crisitem.author.deptSurgery (University of Melbourne)-
crisitem.author.deptSurgery-
crisitem.author.deptHepatopancreatobiliary Surgery-
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