Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/11197
Title: Antibody responses to glycolipid-borne carbohydrates require CD4+ T cells but not CD1 or NKT cells.
Authors: Christiansen, Dale;Vaughan, Hilary A;Milland, Julie;Miland, Julie;Dodge, Natalie;Mouhtouris, Effie;Smyth, Mark J;Godfrey, Dale I;Sandrin, Mauro S
Affiliation: Department of Surgery, The University of Melbourne, Austin Health/Northern Health, Heidelberg, Victoria, Australia.
Issue Date: 1-Feb-2011
Citation: Immunology and Cell Biology 2011; 89(4): 502-10
Abstract: Naturally occurring anti-carbohydrate antibodies play a major role in both the innate and adaptive immune responses. To elicit an anti-carbohydrate immune response, glycoproteins can be processed to glycopeptides and presented by the classical antigen-presenting molecules, major histocompatibility complex (MHC) Class I and II. In contrast, much less is known about the mechanism(s) for anti-carbohydrate responses to glycolipids, although it is generally considered that the CD1 family of cell surface proteins presents glycolipids to T cells or natural killer T (NKT) cells. Using model carbohydrate systems (isogloboside 3 and B blood group antigen), we examined the anti-carbohydrate response on glycolipids using both antibody neutralisation and knockout mouse-based experiments. These studies showed that CD4(+) T cells were required to generate antibodies to the carbohydrates expressed on glycolipids, and unexpectedly, these antibody responses were CD1d and NKT cell independent. They also did not require peptide help. These data provide new insight into glycolipid antigen recognition by the immune system and indicate the existence of a previously unrecognised population of glycolipid antigen-specific, CD1-independent, CD4(+) T cells.
Internal ID Number: 21283109
URI: http://ahro.austin.org.au/austinjspui/handle/1/11197
DOI: 10.1038/icb.2010.166
URL: http://www.ncbi.nlm.nih.gov/pubmed/21283109
Type: Journal Article
Subjects: Animals
Antibody Formation.immunology
Antigens, CD1d.immunology
CD4-Positive T-Lymphocytes.immunology
Glycolipids.immunology
HEK293 Cells
Humans
Mice
Mice, Inbred C57BL
Mice, Knockout
Natural Killer T-Cells.immunology
Rabbits
Appears in Collections:Journal articles

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