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Title: The migration and invasion of human prostate cancer cell lines involves CD151 expression.
Authors: Ang, Jian;Fang, Bing-Liang;Ashman, Leonie K;Frauman, Albert G
Affiliation: Department of Medicine, Clinical Pharmacology and Therapeutics Unit, Austin Health, University of Melbourne, VIC, Australia
Issue Date: 1-Dec-2010
Citation: Oncology Reports; 24(6): 1593-7
Abstract: The molecular mechanisms underlying prostate cancer metastasis remain poorly understood. The tetraspanin family member CD151 has been reported as an 'adaptor' between integrins and signal pathways. The role of CD151 in prostate cancer metastasis in vitro was investigated in this study. LNCap cells were transfected with wild-type CD151 cDNA, mutated CD151 cDNA and vector cDNA. The mutant (QRD194-196 to INF) CD151 cDNA was created using QuickChange 2 site directed Mutagenesis kit (Stratagene). siRNAs were also used to knock down the CD151 expression in the prostate cancer cell line PC3. Proliferation, migration and invasion properties were measured after gene transfection and gene knock-down. There was no difference in proliferation of untransfected or control transfected LNCap cells vs. CD151 transfected LNCap cells (P>0.05). There was greater motility of CD151-transfected vs. control cells, when transferring through migration chambers with or without matrigel-coated membranes (P<0.01, P<0.01). Fewer numbers of mutant-transfected cells were found on the membranes for both migration and invasion studies (P<0.01, P<0.01). CD151 knock-down PC3 cells showed decreased motility (P<0.01), but no change in proliferation (P>0.05). Our data show that CD151 does not change the proliferative properties of prostate cancer cells, but does promote migration and invasion, and suggest that CD151 plays a specific role in promoting prostate cancer cell motility.
Internal ID Number: 21042756
Type: Journal Article
Subjects: Antigens, CD.genetics.metabolism
Antigens, CD151
Cell Adhesion.drug effects.genetics
Cell Line, Tumor
Cell Movement.drug effects.genetics
Cell Proliferation.drug effects
Gene Expression Regulation, Neoplastic.drug effects
Gene Knockdown Techniques
Neoplasm Invasiveness
Prostatic Neoplasms.genetics.pathology
RNA, Small Interfering.pharmacology
Appears in Collections:Journal articles

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